精胺通过降低SAICAr改善ADSL缺陷症的效用研究  

Effect of spermine on ADSL defects by reducing SAICAr

作　　者：黄楠 彭勃[2] 徐蕾[3] 潘武广 赵德志 郭蕾 敖妍冉 朱威 潘秋辉 HUANG Nan;PENG Bo;XU Lei;PAN Wuguang;ZHAO Dezhi;GUO Lei;AO Yanran;ZHU Wei;PAN Qiuhui(Tenth People’s Hospital Affiliated to Tongji University,Shanghai 200072,China;Jinan University,Guangzhou 510632,China;Children’s Medical Center,Shanghai Jiao Tong Universi ty School of Medicine,Shanghai 200127,China;Guangzhou Junh e Biotechnology Co.,LTD.,Guangzhou 510663,China)

机构地区：[1]同济大学附属第十人民医院,上海200072 [2]暨南大学,广东广州510632 [3]上海交通大学医学院附属儿童医学中心,上海200127 [4]广州君赫生物科技有限公司,广东广州510663

出　　处：《当代医药论丛》2023年第10期106-109,共4页

基　　金：广东省海洋经济发展(海洋六大产业)专项资金项目(2021)51号资助(GDNRC[2021]051)。

摘　　要：目的:探讨精胺对琥珀酰氨基咪唑(SAICAr)水平的影响及用于改善腺苷酸琥珀酸裂解酶(ADSL)缺陷症的效用。方法:通过标准品建立了SAICAr检测的质谱技术方法。以5μM精胺处理A549和MDA-MB-231细胞24小时后,收集细胞,通过质谱检测细胞中SAICAr的含量。结论:精胺可有效地降低SAICAr水平,其有望成为ADSL缺陷症治疗领域具有开发前景的新药。Objective:To investigate the effects of spermine on the level of s uccinylaminimidazole(SAICAr)and its effect on improving adenylate succinase(ADSL)defects.Methods:A mass spectrometry technique for SAICAr detection was established by using standard materials.A549 and MDA-MB-231 cells were treated with 5µM spermidine for 24 h,and the SAICAr content in the cells was detected by mass spectrometry.Conclusion:Spermine can effectively reduce the level of SAICAr,and it is expected to be a promising new drug in the treatment of ADSL defects.

关 键 词：精胺 腺苷酸琥珀酸裂解酶缺陷症 琥珀酰氨基咪唑 

分 类 号：R97[医药卫生—药品]