马蔺幼胚高效再生体系的建立  

Establishment of an Efficient Regeneration System in Iris lacteal var.chinensis with Immature Embryo

作　　者：安文杰[1] 王银杰 刘清泉[2] 杨永恒[2] 张婷 范少茹 张永侠[2] 原海燕[2] AN Wenjie;WANG Yinjie;LIU Qingquan;YANG Yongheng;ZHANG Ting;FAN Shaoru;ZHANG Yongxia;YUAN Haiyan(Shanxi Forestry Vocational Technical College,Taiyuan 030009;Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014)

机构地区：[1]山西林业职业技术学院,太原030009 [2]江苏省中国科学院植物研究所,南京210014

出　　处：《植物研究》2023年第3期333-339,共7页Bulletin of Botanical Research

基　　金：中央财政项目(苏[2021]TG04);省级种质资源库项目(2021-SJ-012);科研教研基金资助项目(20200201);国家自然科学基金项目(41907135)。

摘　　要：以马蔺(Iris lacteal var.chinensis)幼胚为外植体,探究不同时期幼胚及不同植物生长调节剂对愈伤组织、体胚和不定芽诱导的影响。结果表明:在本试验条件下最佳取材时间为授粉后35~45 d,此时,幼胚发育完全但胚乳尚未完全硬化,幼胚剥取容易;愈伤组织最适诱导培养基为MS+1.0 mg·L^(-1)2,4-二氯苯氧乙酸(2,4-D)+0.2 mg·L^(-1)6-苄氨基嘌呤(6-BA),诱导率为83.33%;最适体胚诱导培养基为MS+0.25 mg·L^(-1)2,4-D+0.05 mg·L^(-1)6-BA,诱导率71.67%;最佳不定芽诱导和增殖培养基为MS+1.0 mg·L^(-1)噻苯隆(TDZ)+0.2 mg·L^(-1)萘乙酸(NAA),诱导率为78.33%,增殖系数为3.65;在添加0.1 mg·L^(-1)NAA的MS培养基上诱导生根,4周后的生根率达95%以上;生根培养4周的植株清洗掉根部培养基后,移栽至经高温消毒的蛭石中培养,成活率达95%以上。该研究建立了高效的马蔺再生体系,可为马蔺的遗传改良和基因功能研究奠定基础。The effect of sampling time and plant growth regulators on the induction of callus,somatic embryo and adventitious bud were investigated with immature embryos of Iris lacteal var.chinensis as explants.The results showed that the most feasible sampling time for immature embryos was 35-45 d after pollination,the young embryos were fully developed,but the endosperm was not completely hardened,and the young embryo was easy to dissect.The optimum medium for callus induction was MS+1.0 mg·L^(-1)2,4-D+0.2 mg·L^(-1)6-BA with the induction rate of 83.33%.The appropriate medium for somatic cell induction was MS+0.25 mg·L^(-1)2,4-D+0.05 mg·L^(-1)6-BA with the induction rate of 71.67%.The most suitable medium for adventitious bud induction and proliferation was MS+0.2 mg·L^(-1)TDZ+0.05 mg·L^(-1)NAA with the induction rate of 78.33%and proliferation coefficient 3.65 in this experiment.The rooting rate was more than 95%after four weeks cultivation on MS medium supplemented with 0.1 mg·L^(-1)NAA.Then,the medium was washed off and explants were transplanted to sterilized vermiculite,and the survival rate was more than 95%.This study has established an efficient regeneration system of I.lacteal var.chinensis embryos,which can lay a foundation for genetic transformation and gene function analysis of I.lacteal var.chinensis.

关 键 词：马蔺 幼胚 离体培养 愈伤诱导 高效再生 

分 类 号：S682.19[农业科学—观赏园艺]