基于GC-MS分析刺五加和短梗五加不同器官的代谢差异特性  

Analysis of Metabolic Characteristics of Different Organs of Acanthopanax senticosus and Acanthopanax sessiliflorus Based on GC-MS

作　　者：任艳君 孙宇[2] 郭晓瑞[1] 徐明远[3] 邵文达 吴可心 REN Yanjun;SUN Yu;GUO Xiaorui;XU Mingyuan;SHAO Wenda;WU Kexin(College of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin 150040;Heilongjiang Voca-tional Institute Ecological Engineering,Harbin 150025;Heilongjiang University of Chinese Medicine,Harbin 150040;School of Forestry,Northeast Forestry University,Harbin 150040)

机构地区：[1]东北林业大学化学化工与资源利用学院,哈尔滨150040 [2]黑龙江生态工程职业学院,哈尔滨150025 [3]黑龙江中医药大学,哈尔滨150040 [4]东北林业大学林学院,哈尔滨150040

出　　处：《植物研究》2023年第3期439-446,共8页Bulletin of Botanical Research

基　　金：科技部基础资源调查专项项目(2019FY100500);黑龙江省重点研发项目(JD22A008)。

摘　　要：通过研究刺五加(Acanthopanax senticosus)与短梗五加(A.sessiliflorus)不同器官的初级代谢差异特性,探讨两者药效差异、药用价值及资源利用结构。利用代谢组学的研究策略,使用GC-MS技术从初生代谢的角度比较刺五加与短梗五加不同器官代谢差异特性。结果表明:在2种药用植物不同器官中共鉴定出186种初生代谢物,总体研究结果显示2物种在初生代谢上存在差异。进一步分析得到根中52种差异代谢物、茎中34种差异代谢物、叶中39种差异代谢物、叶柄中48种差异代谢物。差异代谢物涉及到的主要代谢途径有氨酰基-tRNA生物合成途径,缬氨酸、亮氨酸和异亮氨酸的生物合成途径,甘氨酸、丝氨酸和苏氨酸代谢途径,精氨酸的生物合成途径,丙氨酸、天冬氨酸和谷氨酸代谢途径,丙酮酸代谢途径等。此外,发现4种连接初生代谢和次生代谢的关键性代谢物在两者不同器官中的水平存在差异。刺五加和短梗五加在不同器官初生代谢方面显著不同,这可能是造成2物种不同器官药理效应和次生代谢存在差异的原因之一。To clarify the differences of primary metabolism between Acanthopanax senticosus and A.sessiliflorus in different organs,and to explore their pharmacodynamic differences,medicinal value,and resource utilization structure,the metabolomics research strategy and GC-MS technology were used as methods.The results showed that 186 primary metabolites were identified in different parts of the two medicinal plants,and there were differences in primary metabolism between the two species.Further analysis showed that 52 differential metabolites in roots,34 differential metabolites in stems,39 differential metabolites in leaves,and 48 differential metabolites in petioles,were obtained respectively.The main metabolic pathways involved in differential metabolites were the aminoacyl tRNA biosynthesis pathway;biosynthetic pathways of valine,leucine,and isoleucine;glycine,serine,and threonine metabolic pathways;biosynthetic pathway of arginine;alanine,aspartate,and glutamate metabolic pathways;pyruvate metabolic pathway,respectively.In addition,there were differences in the levels of four key metabolites connecting primary metabolism and secondary metabolism in different organs respectively.A.senticosus and A.sessiliflorus were significantly different in the primary metabolism of different organs,which might be one of the reasons for the differences in pharmacological effects and secondary metabolism of different organs between the two species.

关 键 词：刺五加 短梗五加 不同器官 初生代谢 GC-MS 多元统计分析 

分 类 号：Q949.763.2[生物学—植物学]