Effects of dietary phosphorus level on growth,body composition,liver histology and lipid metabolism of spotted seabass(Lateolabrax maculatus)reared in freshwater  

作　　者：Jilei Zhang Shuwei Zhang Kangle Lu Ling Wang Kai Song Xueshan Li Chunxiao Zhang Samad Rahimnejad 

机构地区：[1]Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation,Fisheries College,Jimei University,Xiamen,361021,China [2]University of South Bohemia in Ceske Budejovice,Faculty of Fisheries and Protection of Waters,South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses,Zatisi 728/II,Vodnany,38925,Czech Republic

出　　处：《Aquaculture and Fisheries》2023年第5期528-537,共10页渔业学报（英文）

基　　金：This study was supported by National Natural Science Foundation of China(grant number:31972804);the China Agricultural Research System(grant number:CARS47-14).

摘　　要：The present study was conducted to determine the effects of dietary phosphorus(P)levels on growth performance,body composition,liver histology and enzymatic activity,and expression of lipid metabolism-related genes in spotted seabass(Lateolabrax maculatus).Seven diets were prepared to contain available P levels of 0.48%(the control group),0.69%,0.89%,1.10%,1.28%,1.51%and 1.77%and feed fish(4.26±0.03 g)to satiety twice daily for 10 weeks.Significantly higher weight gain and specific growth rate were recorded at P levels of 0.69%-1.51%compared to the control group.Feed conversion ratio decreased with increasing P levels up to 0.89%and increased thereafter.The lowest liver lipid content,viscerosomatic index and lipid content of whole-body were obtained in the 0.89%-P group among dietary treatments.P and calcium(Ca)contents in whole body were increased,while liver triglyceride and cholesterol contents were decreased with increasing dietary P levels from 0.48%to 1.77%.The highest activity of hepatic lipase was recorded in the 1.10%-P group among dietary treatments.Compared to the control group,1.10%P enhanced the proportion of HUFA and reduced the proportion of SFA and MUFA.The histological observations showed that P deficiency(0.48%)led to the vacuolization of hepatocytes and increased number of lipid droplets.Meanwhile,overall liver tissue structure was improved when P level increased to 1.28%.Compared to the control group,expression of lipid metabolism-related genes such as FAS,ACC-2 and SREBP-1 was decreased at 0.89%-1.10%P group while an opposite trend was observed in the expression of PPARa2 and CPT-1 genes.The current study showed that 0.89%dietary P levels could promote growth performance of spotted seabass and reduce lipid accumulation in the liver.A broken-line regression analysis based on weight gain showed that the optimum dietary P level(available P)for juvenile spotted seabass reared in freshwater was 0.72%.

关 键 词：Lateolabrax maculatus Phosphorus requirement Lipid metabolism GROWTH Liver histology Liver fatty acid composition 

分 类 号：S917.4[农业科学—水产科学]