端粒酶逆转录酶在建立永生化细胞中的应用  

Application of Telomerase Reverse Transcriptase in Establishment of Immortalized Cells

作　　者：赵梦坡 八晓敏 罗胜军 向华 王晓虎 ZHAO Meng-po;BA Xiao-min;LUO Sheng-jun;XIANG Hua;WANG Xiao-hu(Institute of Animal Health,Guangdong Academy of Agricultural Sciences,Key Laboratory of Livestock Disease Prevention of Guangdong Province,Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province,Ministry of Agriculture and Rural Areas,Guangzhou 510640,China;College of Veterinary Medicine,South China Agricultural University,Guangzhou 510640,China)

机构地区：[1]广东省农业科学院动物卫生研究所,广东省畜禽疫病防治研究重点实验室,农业农村部兽用药物与诊断技术广东科学观测实验站,广东广州510640 [2]华南农业大学兽医学院,广东广州510600

出　　处：《动物医学进展》2023年第5期102-107,共6页Progress In Veterinary Medicine

基　　金：“十四五”国家重点研发计划项目(2021YFD1801402);广东省自然科学基金项目(2020A1515011112);广东省非洲猪瘟科技应急防控专题项目(20190211)。

摘　　要：永生化细胞是研究细胞如何增殖、分化、凋亡过程及细胞衰老现象等机理的理想细胞模型。研究建立有效促进正常细胞永生化的生物学方法,相对于由某些基因突变、理化刺激及病毒、原癌基因直接诱导刺激等方法构建的永生化细胞系,应用端粒酶逆转录酶(telomerase reverse transcriptase, TERT)重新激活端粒酶活性来维持细胞增殖能力的方法被证明是安全有效的。论文阐述了近些年TERT所构建的永生化细胞系在组织工程、临床治疗、基础研究等方面的应用,总结了TERT构建的永生化细胞系对人和动物疾病相关研究起到的作用,并对其在细胞农业的应用价值进行了展望。Immortalized cell is an ideal cell model to study the mechanism of cell proliferation,differentiation,apoptosis and cell aging,which is conducive to basic research and bioengineering.Over the years,scholars have established a variety of biological methods to effectively promote the immortalization of normal cells.Compared with the immortalized cell lines constructed by some gene mutation,physical and chemical stimulation,and direct induction and stimulation of viruses and protooncogenes,the method of using telomerase reverse transcriptase(TERT)to reactivate telomerase activity to maintain cell proliferation has been proved to be safe and effective.In this paper,the applications of immortalized cell lines constructed by TERT in tissue engineering,clinical treatment and basic research in recent years are described,the role of immortalized cell lines constructed by TERT in the research of human and animal diseases is summarized,and its application value in cell agriculture is prospected.

关 键 词：端粒 端粒酶逆转录酶 细胞 永生化 

分 类 号：Q813.5[生物学—生物工程]