内蒙古规模化羊场羊流产病原与抗体的检测分析  

Detection and Analysis of Pathogens and Antibodies of Sheep Abortion in Large-scale Sheep Farms in Inner Mongolia

作　　者：郑红飞 张成 泽让卓玛 曹小安[1] 张登基 卢旺银[2] 尚佑军[1] ZHENG Hong-fei;ZHANG Cheng;ZERANG Zhuo-ma;CAO Xiao-an;ZHANG Deng-ji;LU Wang-yin;SHANG You-jun(Lanzhou Veterinary Research Institute,Chinese Academy of AgricμLtural Sciences,State Key Laboratory of Veterinary Etiologic Biology,Lanzhou,Gansu,730046,China;Gansu Provincial Animal Disease Prevention and Control Center,Lanzhou,Gansu,730046,China)

机构地区：[1]中国农业科学院,兰州兽医研究所,家畜疫病病原生物学国家重点实验室,甘肃兰州730046 [2]甘肃省动物疫病预防控制中心,甘肃兰州730046

出　　处：《动物医学进展》2023年第5期122-127,共6页Progress In Veterinary Medicine

基　　金：2021年度宁夏自治区重点研发计划重点课题(2021BEF02026);国家肉羊产业技术体系项目(CARS-39-04B)。

摘　　要：为了诊断引起内蒙古羊场怀孕母羊流产的病原因素,通过对流产母羊血清、流产胎儿和胎衣等样本进行抗体检测、病理剖检、PCR扩增、核苷酸序列比对、同源性分析和构建系统进化树等方法进行检测分析。结果布鲁氏菌检出率最高(34.28%),流产衣原体次之(28.57%),伯氏柯克氏体与布鲁氏菌的混合感染和3种病原体的混合感染检出率最低(2.86%)。说明检测羊场中均存在流产衣原体、伯氏柯克氏体和布鲁氏菌单独感染或混合感染的情况,且3种病原体保守性较高,为检测羊场及周边其他羊场疫病防控提供了科学依据。In order to diagnose the etiology causing abortion in pregnant ewes in Inner Mongolia sheep farms,this paper was conducted by antibody detection,pathological dissection,PCR amplification,nucleotide sequence comparison,homology analysis and construction of phylogenetic trees on samples of aborted ewes'sera,aborted fetuses and fetal coats.The resμLts showed the highest detection rate of Brucella abortus(34.28%),followed by Chlamydia abortus(28.57%),and the lowest detection rate of mixed infection of Q fever and Brucella abortus and mixed infection of three pathogens(2.86%).This indicates that Chlamydia abortus,Q fever and Brucella abortus are all present in the tested sheep farms as separate or mixed infections,and the conservativeness of the three pathogens is high,which provides a scientific basis for the detection of disease prevention and control in sheep farms and other surrounding sheep farms.

关 键 词：流产衣原体 伯氏柯克氏体 布鲁氏菌 混合感染 

分 类 号：S852.67[农业科学—基础兽医学] S858.26[农业科学—兽医学]