华中五味子果实化学成分的分离鉴定及其抗糖尿病活性研究  

Chemical constituents from fruits of Schisandra sphenanthera and their anti-diabetic activities

作　　者：李斌 萧琪 张嘉诺 刘久石 张本刚[1] 刘海涛[1] LI Bin;XIAO Qi;ZHANG Jia-nuo;LIU Jiu-shi;ZHANG Ben-gang;LIU Hai-tao(Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education,Institute of Medicinal Plant Development&Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100193,China)

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,中草药物质基础与资源利用教育部重点实验室,北京100193

出　　处：《中草药》2023年第8期2379-2387,共9页Chinese Traditional and Herbal Drugs

基　　金：中央高校基本科研业务费专项资金资助(3332021049);中国医学科学院医学与健康科技创新工程项目(2021-I2M-1-031)。

摘　　要：目的研究华中五味子Schisandra sphenanthera果实的化学成分及其抗糖尿病活性。方法运用硅胶、Sephadex LH-20、半制备HPLC等手段对其醇提物进行分离纯化,利用^(1)H-NMR、^(13)C-NMR、MS等波谱技术鉴定所得化合物结构。采用高脂饮食诱导的糖尿病小鼠模型,通过ip葡萄糖耐量实验、ip胰岛素耐量实验及血清中总胆固醇(total cholesterol,TC)、三酰甘油(triglyceride,TG)、胰岛素(insulin,INS)水平检测,对化合物2进行抗糖尿病活性研究。结果从华中五味子果实中分离得到19个化合物,分别鉴定为戈米辛J(1)、kadsurarin(2)、前戈米辛(3)、戈米辛O(4)、arisanschinin G(5)、sieverlignan E(6)、五味子酯甲(7)、异型南五味子庚素(8)、kadoblongifolin C(9)、五味子酯丁(10)、五味子酚(11)、schizanrin F(12)、7(18)-dehydroschisandro A(13)、戈米辛M1(14)、安五脂素(15)、桉脂素(16)、kadsufolin A(17)、7-乙氧基迷迭香酚(18)、3,3’-bisdemethylpinoresinol(19)。其中,化合物2可有效改善糖尿病小鼠糖耐量和胰岛素抵抗,降低血清中TC、TG、INS水平。结论化合物6、8~9、16~19为首次从五味子属植物中分离得到,化合物2、5、12~13为首次从该植物中发现。化合物2具有较好的抗糖尿病活性。Objective To isolate and identify the chemical constituents with anti-diabetic activities from the ethanol extract of Schisandra sphenanthera fruits.Methods The isolation and purification were performed by silica gel,Sephadex LH-20 gel,and semi-preparative high performance liquid chromatography.Their structures were elucidated by ^(1)H NMR,^(13)C NMR,and MS.The anti-diabetic effect of compound 2 was evaluated by the intraperitoneal glucose tolerance test(IPGTT),intraperitoneal insulin tolerance test(IPITT),and serum total cholesterol(TC),triglyceride(TG)and insulin(INS)levels in a high-fat diet(HFD)-induced diabetic mice model.Results A total of 19 compounds were isolated from the ethanol extract of S.sphenanthera fruits.Their structures were identified as gomisin J(1),kadsurarin(2),pregomisin(3),gomisin O(4),arisanschinin G(5),sieverlignan E(6),schisantherin A(7),heteroclitin G(8),kadoblongifolin C(9),schisantherin D(10),schisanhenol(11),schizanrin F(12),7(18)-dehydroschisandro A(13),gomisin M1(14),anwulignan(15),eudesmin(16),kadsufolin A(17),7-ethoxyrosmanol(18),3,3′-bisdemethylpinoresinol(19).In vivo experiments demonstrated that 2 treatments potently alleviated glucose tolerance and insulin resistance,and reduced the levels of serum TG,TC,and INS.Conclusion Compounds 6,8—9,and 16—19 are isolated from genus Schisandra for the first time,and compounds 2,5,and 12—13 are found for the first time from this plant.Compound 2 showed a good anti-diabetic activity.

关 键 词：华中五味子 木脂素 二萜 抗糖尿病 kadsurarin 异型南五味子庚素 桉脂素 7-乙氧基迷迭香酚 

分 类 号：R284.1[医药卫生—中药学]