掌叶大黄转录组测序及蒽醌类合成关键酶基因差异表达分析  

Transcriptome sequencing and expression analysis of anthraquinone synthesis key enzyme genes on Rheum palmatum

作　　者：邹建珍 胡晓晨 李依民[2] 郝紫彤 张岗 刘蒙蒙[1] ZOU Jian-zhen;HU Xiao-chen;LI Yi-min;HAO Zi-tong;ZHANG Gang;LIU Meng-meng(College of Traditional Chinese Medicine,Hebei University,Baoding 071002,China;Key Laboratory for Research and Development of“Qin Medicine”of Shaanxi Administration of Traditional Chinese Medicine,College of Pharmacy,Shaanxi University of Chinese Medicine,Xi’an 712046,China)

机构地区：[1]河北大学中医学院,河北保定071002 [2]陕西中医药大学药学院/陕西省中医药管理局“秦药”研发重点实验室,陕西西安712046

出　　处：《中草药》2023年第7期2217-2225,共9页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(82104334);国家自然科学基金资助项目(82204567);国家自然科学基金资助项目(8220142901);陕西中医药大学“秦药”品质评价与资源开发创新团队项目(2019-QN01);咸阳市中青年科技创新领军人才(L2022CXNLRC009)。

摘　　要：目的研究掌叶大黄Rheum palmatum蒽醌合成关键基因。方法利用RNA-seq技术对掌叶大黄根、根茎、叶进行转录组测序和生物信息学分析。结果经Trinity软件组装后获得140224条Unigenes,全部Unigenes能被非冗余蛋白库、核酸序列数据库、京都基因组百科全书数据库、蛋白质序列数据库、蛋白家族数据库、基因本体联合数据库、同源蛋白簇数据库等公共数据库注释。差异基因分析结果显示,掌叶大黄的根与叶相比,差异表达基因总数为4175个,根与根茎相比,差异表达基因总数为992个,叶与根茎相比,差异表达基因总数为4469个。差异表达基因代谢途径分析分析发现,苯丙烷生物合成通路在叶比根中被显著富集。蒽醌类化合物合成相关关键差异表达基因分析发现,关键差异表达基因主要涉及甲羟戊酸(mevalonic acid,MVA)途径、甲基赤藓糖醇(methylerythritol 4-phosphate,MEP)、莽草酸及聚酮途径的13个基因。结论为进一步挖掘大黄有效成分生物合成过程中的关键基因,为解析调控其有效成分生物合成途径提供研究基础。Objective To identify genes involved in anthraquinones biosynthesis in Rheum palmatum.Methods RNA-seq was used to investigate the gene expression profiles of leaves,rhizome,and root from R.palmatum.Results A total of 140224 unigenes were obtained through the Trinity assembling.Functional annotation revealed that all unigenes were successfully annotated in the NR,NT,Swiss-port,PFAM,and KOG databases.Differentially expressed genes(DEGs)analysis suggested that 4175992 and 4469 genes were differentially expressed in root/leaf,root/rhizome and leaf/rhizome,respectively.KEGG analysis showed that the Phen propane biosynthesis pathway was significantly enriched in leaf/root.The analysis of key differentially expressed genes related to anthraquinone synthesis revealed 13 genes mainly involved in the mevalonic acid(MVA)pathway,methylerythritol 4-phosphate(MEP),mangiferous acid and polyketide pathways.Conclusions The key genes are further explored in the biosynthesis of the active ingredients of rhubarb in order to provide a research basis for the analysis of the pathways regulating the biosynthesis of its active ingredients.

关 键 词：掌叶大黄 蒽醌 转录组 基因表达 甲羟戊酸途径 甲基赤藓糖醇途径 

分 类 号：R286.12[医药卫生—中药学]