系统性红斑狼疮患者血浆METTL3、WTAP、FTO水平变化及与临床指标的相关性  

Changes of plasma METTL3,WTAP and FTO levels in patients with systemic lupus erythematosus and their correlation with clinical indicators

作　　者：刘瑜[1] 唐莼 雷艳[1] 郑智华[1] Liu Yu;Tang Chun;Lei Yan;Zheng Zhihua(Center of Kidney and Urology,the Seventh Affiliated Hospital,Sun Yat-sen University,Shenzhen 518107,China)

机构地区：[1]中山大学附属第七医院肾脏病中心,深圳518107

出　　处：《中国医师杂志》2023年第4期546-550,共5页Journal of Chinese Physician

基　　金：广东省基础与应用基础研究基金(2019A1515110488,2020A1515110306,2020A1515111209);深圳市基础研究资助项目(JCYJ20210324123414040);深圳三名工程(SZSM201911013)。

摘　　要：目的通过比较系统性红斑狼疮(SLE)患者与健康对照组之间血浆N6-甲基腺嘌呤(m6A)修饰相关蛋白[甲基化转移酶3(METTL3)、甲基化转移酶14(METTL14)、肾母细胞瘤1关联蛋白(WTAP)、AlkB同源蛋白5(ALKBH5)、肥胖相关蛋白(FTO)]和m6A水平变化,探讨m6A在SLE中的临床意义。方法共选取64例SLE患者和24例健康志愿者,比较两组血浆METTL3、METTL14、WTAP、ALKBH5、FTO和m6A水平,分析METTL3、WTAP、FTO水平与临床指标的相关性。结果SLE患者血浆METTL3水平明显高于对照组(P<0.05),血浆WTAP和FTO水平明显低于对照组(均P<0.05)。SLE患者血浆METTL3水平与血红蛋白水平呈负相关(r=-0.344,P<0.05);血浆FTO水平与血浆IgM水平呈正相关(r=0.337,P<0.05),与血浆IgA水平呈负相关(r=-0.286,P<0.05)。高水平METTL3组SLE患者肾脏累及的发生率、血浆抗组蛋白抗体阳性率更高(均P<0.05)。低水平FTO组SLE患者血浆抗dsDNA抗体、抗Sm抗体及AuaA自身抗体阳性率更高(均P<0.05)。结论SLE患者血浆METTL3水平明显升高,血浆WTAP和FTO水平明显降低,并与多种临床指标相关,与SLE发病可能存在一定联系。Objective To explore the clinical significance of N6-methyladenine(m6A)in systemic lupus erythematosus(SLE)by comparing the changes in plasma levels of m6A modification related proteins[methyltransferase 3(METTL3),methyltransferase 14(METTL14),Wilms tumor 1 associated protein(WTAP),AlkB homologous protein 5(ALKBH5),and fat mass and obesity-associated protein(FTO)]and m6A between patients with systemic lupus erythematosus(SLE)and healthy controls.Methods A total of 64 SLE patients admitted to the Seventh Affiliated Hospital of Sun Yat-Sen University from May 2020 to June 2022 and 24 healthy volunteers during the same period were selected to compare and analyze the plasma levels of METTL3,METTL14,WTAP,ALKBH5,FTO and m6A between the two groups.The correlation between METTL3,WTAP,FTO levels and clinical indicators was analyzed.Results The plasma METTL3 level of SLE patients was significantly higher than that of control group(P<0.05),and the plasma WTAP and FTO levels were significantly lower than those of control group(all P<0.05).In SLE patients,plasma METTL3 level was negatively correlated with hemoglobin level(r=-0.344,P<0.05),plasma FTO level was positively correlated with plasma IgM level(r=0.337,P<0.05),and plasma IgA level was negatively correlated with SLE patients(r=-0.286,P<0.05).The incidence of renal involvement and positive rate of plasma anti-histone antibody were higher in SLE patients with high METTL3 level(all P<0.05).The positive rates of plasma anti-dsDNA antibody,anti-SM antibody and AuaA antibody were higher in SLE patients with low FTO level(all P<0.05).Conclusions The plasma METTL3 level in SLE patients are significantly increased,while the plasma WTAP and FTO levels are significantly reduced,which are related to various clinical indicators and may be related to the onset of SLE.

关 键 词：红斑狼疮 系统性 甲基转移酶样蛋白3 肾母细胞瘤1关联蛋白 肥胖相关蛋白 

分 类 号：R593.241[医药卫生—内科学]