Fluorescence immunoassay based on alkaline phosphatase-induced in situ generation of fluorescent non-conjugated polymer dots  

作　　者：Donghui Wu Qilin Zhao Jian Sun Xiurong Yang 

机构地区：[1]State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China [2]School of Applied of Chemistry and Engineering,University of Science and Technology of China,Hefei 230026,China

出　　处：《Chinese Chemical Letters》2023年第3期231-235,共5页中国化学快报（英文版）

基　　金：financial supports of the National Natural Science Foundation of China(Nos.22034006,21974132 and 21721003);the Youth Innovation Promotion Association,CAS(No.2018258);the Development Project of Science and Technology of Jilin Province,China(No.20200201091JC)。

摘　　要：Alkaline phosphatase(ALP) activity assay is not only significant to the clinical diagnosis of some related disease, but also momentous to the construction of ALP-based enzyme-linked immunosorbent assay(ELISA). Herein, for the first time, we have discovered that ascorbic acid(AA) can specially react with N-methylethylenediamine(N-MEDA) to generate fluorescent non-conjugated polymer dots(NCPDs) under mild conditions. On the basis of the AA-responsive emission and ALP-catalyzed hydrolysis of ascorbic acid 2-phosphate(AA2P) to AA, we have exploited a fluorometric ALP activity assay with high sensitivity and selectivity. Furthermore, by means of conventional ALP-based ELISA platform, a conceptual fluorescent ELISA has been constructed and applied in the potential clinical diagnosis, during which cardiac troponin I(cTnI), a well-established biomarker of acute myocardial infarction, has been chosen as the model target. We envision that such original fluorescent NCPDs generation-enabled ELISA could become a versatile tool in biochemical sensing and medical diagnosis in the future.

关 键 词：Alkaline phosphatase Non-conjugated polymer dots Fluorescent sensor Fluorescent ELISA Cardiac troponin I 

分 类 号：Q503[生物学—生物化学]