Discovering ester and ether derivatives of luminol as advanced chemiluminescence probes  

作　　者：Fuqian Chen Ying Zhang Ting Li Danfeng Peng Zhiwen Qi Jianping Song Tao Deng Fang Liu 

机构地区：[1]Artemisinin Research Center,Guangzhou University of Chinese Medicine,Guangzhou 510006,China [2]School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006,China [3]Lingnan Medical Research Center,The first Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510006,China [4]Institute of Chemical Industry of Forest Products,Chinese Academy of Forestry(CAF),Nanjing 210042,China

出　　处：《Chinese Chemical Letters》2023年第3期264-268,共5页中国化学快报（英文版）

基　　金：projects of the Department of Education of Guangdong Province(Nos.2021ZDZX2030 and 2020ZDZX2017);Guangdong Basic and Applied Basic Research Foundation(No.2021A1515110115);Guangzhou Basic and Applied Basic Research Foundation(No.202102020548)for funding support。

摘　　要：A variety of luminol derivatives with N-and O-substitution have been synthesized with broad functional group tolerance. O-esterification has been demonstrated for the first time as a promising way to prepare enhanced CL reagents for sensing hemin, bloodstain and horseradish peroxidase(HRP). The most effective analogue with a deuterated acetyl group exhibited greater potential than luminol for bloodstain imaging and HRP imaging in western blotting(WB). In addition, O-etherification can greatly suppress CL signal that has been applied to design a CL probe for β-glucosidase(β-Glu). This study offers important and useful information regarding the luminol modification and shows great potential to use O-substituted analogues for enhanced CL analysis.

关 键 词：Luminol derivatives Chemiluminescence(CL) Luminol O-modification Bloodstain imaging WB imaging reagent 

分 类 号：R313[医药卫生—基础医学] O657.3[理学—分析化学]