Highly lipophilic coumarin fluorophore with excimer-monomer transition property for lipid droplet imaging  

作　　者：Xiang Shi Ge Gao Xiaoyang Liu Lingling Xu Yu Deng Rui Wang Gaolin Liang 

机构地区：[1]State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China

出　　处：《Chinese Chemical Letters》2023年第3期367-371,共5页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.21725505,22074016 and 81821001)。

摘　　要：Lipid droplet(LD) fluorescent imaging plays an important role in the detection of lipid-related diseases.Due to their poor photostability and low hydrophobicity of currently available LD imaging fluorophores,LD imaging is limited by its short imaging period and low imaging contrast. Herein, we reasonably designed a highly lipophilic compound Cou-Flu with excellent photostability and excimer-monomer transition property. It exhibited weak excimer emission in cytoplasm, but strong monomer emission in LDs,enabling high contrast LD imaging and LD movement tracing in cells. Zebrafish imaging study demonstrated that Cou-Flu was also suitable for in vivo LD detection with excellent sensitivity. We anticipate that Cou-Flu could be widely applied to understand LD-related intracellular activities and even LD-related diseases in the future.

关 键 词：Excimer-monomer transition Fluorescent probe Hydrophobicity Lipid droplets Monomer emission 

分 类 号：O657.3[理学—分析化学] Q25[理学—化学]