骨髓间充质干细胞治疗皮肤深Ⅱ度烧伤的实验研究  

Experimental Study on Bone Marrow Mesenchymal Stem Cells in Treatment of Deep Second Degree Skin Burn

作　　者：周小龙 吴奔 李弘德 Zhou Xiao-long;Wu Ben;Li Hong-de(Changsha Medical College,Changsha 410219,Hunan Province,China;Wuzhou Medical College,Wuzhou 543002,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]长沙医学院,湖南长沙410219 [2]梧州医学高等专科学校,广西梧州543002

出　　处：《中国社区医师》2023年第12期11-13,共3页Chinese Community Doctors

基　　金：广西教育厅广西高校中青年教师科研基础能力提升项目(编号:2021KY1962);湖南省教育厅优秀青年项目(编号:20B073)。

摘　　要：目的:分析在皮肤深Ⅱ度烧伤治疗中应用骨髓间充质干细胞的效果。方法:选取2020年4月—2021年8月试验动物中心购入的50只仓鼠作为研究对象,采用随机数字表法分为对照组与观察组,各25只。观察组采用骨髓间充质干细胞处理烧伤部位,对照组采用等量生理盐水处理创面,对比两组创面愈合面积、皮肤肥大细胞糜蛋白酶mRNA表达水平。结果:治疗3 d、1周、2周时,观察组创面愈合面积大于对照组,差异有统计学意义(P=0.001)。治疗1 d、3 d、1周、2周时,两组均测出糜蛋白酶基因;观察组治疗1周时,mRNA测定水平最高;治疗1、2周时,观察组糜蛋白酶mRNA水平高于对照组,差异有统计学意义(P=0.001)。结论:骨髓间充质干细胞治疗皮肤深Ⅱ度烧伤,可促进烧伤创面的愈合。Objective:To analyze the application effect of bone marrow mesenchymal stem cells in the treatment of deep second degree skin burn.Methods:A total of 50 hamsters purchased in Experimental Animal Center from April 2020 to August 2021 were selected as the study objects.They were divided into control group and observation group according to random number table method,with 25 hamsters in each group.The burn wounds in the observation group were treated with bone marrow mesenchymal stem cells and the wounds in the control group were treated with the same amount of physiological saline.The wound healing area and skin mast cell chymotrypsin mRNA expression levels were compared between two groups.Results:After 3 d,1 week and 2 weeks of treatment,the wound healing area in the observation group was larger than that in the control group,and the difference was statistically significant(P=0.001).After 1 d,3 d,1 week and 2 weeks of treatment,chymotrypsin gene was detected in both groups;After 1 week of treatment,the mRNA assay level was highest in the observation group;After 1 week and 2 weeks of treatment,the level of chymotrypsin mRNA in the observation group was higher than that in the control group,and the difference was statistically significant(P=0.001).Conclusion:The bone marrow mesenchymal stem cells in the treatment of deep second degree skin burn can promote the healing of burn wounds.

关 键 词：皮肤深Ⅱ度烧伤 骨髓间充质干细胞 愈合面积 肥大细胞糜蛋白酶 

分 类 号：R644[医药卫生—外科学]