小鼠非手术胚胎移植器及移植技术改进  

Improving the transcervical embryo transfer devices and transfer technology of mice

作　　者：王苗苗 王浩洋 张静 陆晓雨 李桂琳 李相运[1] Wang Miaomiao;Wang Haoyang;Zhang Jing;Lu Xiaoyu;Li Guilin;Li Xiangyun(College of Animal Science and Technology,Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北农业大学动物科技学院,保定071000

出　　处：《解剖学杂志》2023年第1期15-19,共5页Chinese Journal of Anatomy

基　　金：河北省重点研发项目(20326610D)。

摘　　要：目的 :提高小鼠非手术法胚胎移植成功率,改进小鼠的非手术法胚胎移植器,完善小鼠非手术法胚胎移植程序。方法 :设计3种不同的胚胎移植器,比较不同胚胎移植器的移植成功率,进一步改进移植器,优化移植程序。结果 :C型移植器的移植妊娠率和着床率(77.8%、60.3%)均高于A型(66.7%、23.0%)和B型(61.1%、45.1%)。将C型移植器移植管的长度延长至27mm,移植后的妊娠率、着床率都得到进一步提高,分别为92.9%、76.9%,出生率也达到73.1%。结论 :本研究成功改进了小鼠非手术胚胎移植器,优化了移植程序,使非手术法胚胎移植效率与手术法相当,且更加简单、快捷、易掌握。Objective:In order to increase the success rate of non-surgical embryo transfer,improve the nonsurgical embryo transfer device and optimize the procedure of non-surgical embryo transfer in mice.Methods:Three different transcervical embryo transer devices were designed in this study,and the procedure of non-surgical embryo transfer in mice was optimized by comparing the success rate of different transcervical embryo transfer devices.Results:The pregnancy rate and implantation rate of type C(77.8%and 60.3%)were higher than those of type A(66.7%and 23.0%)and type B(61.1%and 45.1%).Therefore,we further improved the type C by extending the length of the tube to 27 mm,and the pregnancy rate and implantation rate after embryo transfer were further improved,which were 92.9%and 76.9%respectively,and the birth rate also reached 73.1%.Conclusion:This study has successfully improved the mice transcervical embryo transfer device,optimized the transfer procedure,made the transcervical embryo transfer as efficient as surgical embryo transfer,which is much simpler,faster,and easier to use.

关 键 词：非手术移植器 胚胎移植 妊娠率 着床率 小鼠 

分 类 号：R714.8[医药卫生—妇产科学]