长链非编码RNA MicroRNA155宿主基因和微RNA-128-5p在慢性阻塞性肺疾病病人中的表达  

Expression of LncRNA MIR155HG and miR-128-5p in patients with chronic obstructive pulmonary disease

作　　者：李慧 赵亚光 陈奇 赵十妹 靳杨 李颖 LI Hui;ZHAO Yaguang;CHEN Qi;ZHAO Shimei;JIN Yang;LI Ying(Department of Respiratory Medicine,Beijing First Hospital of Integrated Chinese and Western Medicine,Beijing 100026,China)

机构地区：[1]北京市第一中西医结合医院呼吸内科,北京100026

出　　处：《安徽医药》2023年第6期1199-1203,I0009,共6页Anhui Medical and Pharmaceutical Journal

摘　　要：目的 探讨长链非编码RNA(LncRNA) MicroRNA155宿主基因(MIR155HG)、微RNA(miR)-128-5p水平与慢性阻塞性肺疾病(COPD)病人的关系及临床意义。方法 选取2019年5月至2021年5月北京市第一中西医结合医院收治的COPD病人100例,其中急性加重期COPD病人为A组(n=52),稳定期COPD病人为B组(n=48),选取同期来该院体检且与病人一般资料匹配的健康人群为C组(n=50)。采用实时荧光定量PCR(qRT-PCR)法检测血清LncRNA MIR155HG、miR-128-5p表达水平;Pearson法分析COPD病人血清LncRNA MIR155HG与miR-128-5p表达水平的相关性;受试者操作特征(ROC)曲线分析血清LncRNA MIR155HG、miR-128-5p水平诊断急性加重期COPD病人的价值。结果 COPD加重期和稳定期病人血清中LncRNA MIR155HG(3.59±0.82、2.35±0.55)与miR-128-5p(0.33±0.11、0.51±0.12)的表达相较于健康人群(1.06±0.27、0.95±0.13)中升高,差异有统计学意义(F=230.26、356.75,P<0.05);COPD病人血清中肿瘤坏死因子-α(TNF-α)、降钙素原、超敏C反应蛋白(hsCPR)、白细胞水平均上升,第一秒用力呼吸容积(FEV1)%、FEV1/用力肺活量(FVC)水平均下调,差异有统计学意义(P<0.05);血清中LncRNA MIR155HG与TNF-α、降钙素原、hs-CPR、白细胞呈正相关,与FEV1%、FEV1/FVC呈负相关(P<0.05);miR-128-5p与TNF-α、降钙素原、hs-CRP、白细胞呈负相关,与FEV1%、FEV1/FVC呈正相关(P<0.05);COPD病人血清LncRNA MIR155HG和miR-128-5p表达呈明显负相关(r=-0.49,P<0.05);ROC结果显示,LncRNA MIR155HG、miR-128-5p二者联合诊断急性加重期COPD病人的曲线下面积(AUC)为0.98,95%CI:(0.93,1.00),显著高于LncRNA MIR155HG、miR-128-5p单独诊断。结论 LncRNA MIR155HG在COPD病人血清中明显高表达,miR-128-5p呈低表达,二者呈明显负相关,且与病情严重程度密切相关,二者联合检测对评估急性加重期COPD病人的临床价值高于单一指标,可作为诊断急性加重期COPD病人的潜在血清标志物。Objective To investigate the relationship between and clinical significance of the long noncoding RNA(lncRNA)MIR155HG and miR-128-5p levels in patients with chronic obstructive pulmonary disease(COPD).Methods A total of 100 COPD patients admitted to Beijing First Integrated Traditional Chinese and Western Medicine Hospital from May 2019 to May 2021 were selected.Among them,patients with acute exacerbation COPD were group A(n=52),patients with stable COPD were group B(n=48),and healthy people who came to our hospital for physical examination during the same period and matched with patients'general information were selected as group C(n=50).Quantitative real-time fluorescent quantitative PCR(qRT‒PCR)was used to detect the expression levels of serum lncRNA MIR155HG and miR-128-5p.Pearson's method was used to analyze the correlation between the expression levels of serum lncRNA MIR155HG and miR-128-5p in COPD patients.Receiver operating characteristic curve(ROC)analysis of the value of serum lncRNA MIR155HG and miR-128-5p levels in diagnosing patients with acute exacerbation of COPD.Results The expressions levels of lncRNA MIR155HG(3.59±0.82,2.35±0.55)and miR-128-5p(0.33±0.11,0.51±0.12)were elevated in the serum of exacerbation and stable COPD patients compared to the healthy population(1.06±0.27,0.95±0.13),with statistically significant differences(F=230.26,356.75,P<0.05).The serum levels of tumor necrosis factor-α(TNF-α),calcitoninogen(PCT),hypersensitive C-reactive protein(hs-CPR),and white blood cells(WBC)were increased,and the levels of forced expiratory volume in one second(FEV1)%and FEV1/forced vital capacity(FVC)were downregulated in COPD patients,with statistically significant differences(P<0.05).The serum lncRNA MIR155HG was positively correlated with TNF-α,PCT,hs-CPR,and WBC count,and negatively correlated with FEV1%and FEV1/FVC(P<0.05),while miR-128-5p was negatively correlated with TNF-α,PCT,hs-CRP,and WBC count,and positively correlated with FEV1%and FEV1/FVC(P<0.05).The expression of serum

关 键 词：肺疾病 慢性阻塞性 长链非编码RNA MicroRNA155宿主基因 相关性 肺功能 肿瘤坏死因子Α C反应蛋白质 用力呼气量 

分 类 号：R563.9[医药卫生—呼吸系统]