冷冻电镜含水切片技术观察胰岛β细胞的三维超微结构  

3D structure of βcells in mouse islets under Cryo-electron microscopy of vitreous sections

作　　者：王培 冯凤萍 董宜君 李霞[3] 薛艳红[1] WANG Pei;FENG Feng-ping;DONG Yi-jun;LI Xia;XUE Yan-hong(National Laboratory of Biomacromolecules,CAS Center for Excellence in Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101;Laiwu No.l Senior High School,Jinan Shandong 271100;Institute of Special Environmental Medicine,Nantong University,Nantong Jiangsu 226000,China)

机构地区：[1]中国科学院生物物理研究所生物大分子国家重点实验室,北京100101 [2]山东省济南市莱芜第一中学,山东济南271100 [3]南通大学特种医学研究院,江苏南通226000

出　　处：《电子显微学报》2023年第2期186-191,共6页Journal of Chinese Electron Microscopy Society

基　　金：国家重点研发计划(No.2017YFA0504700)。

摘　　要：冷冻电镜含水切片技术(CEMOVIS)作为冷冻厚生物样品制备技术,对高压冷冻的样品直接进行冷冻切片,能够获得完全含水状态的生物样品的超微结构,更好地反映出细胞接近其生理条件的真实状态。本文应用冷冻含水切片技术结合冷冻电子断层扫描和三维重构技术观察到了小鼠胰岛β细胞内细胞器的高分辨超微结构。After high pressure freezing and sectioning on thick specimen,Cryo-electron microscopy of vitreous sections(CEMOVIS)can acquire ultrastructure of vitreous samples close to their physiological states.Here,we applied CEMOVIS on mouse islets,and reconstructed the ultrastructure of the cell organelles in their native states at high resolution.Our work paves the way to study the native ultrastructure of mammalian tissues via CEMOVIS.

关 键 词：高压冷冻 冷冻含水切片 胰岛Β细胞 冷冻电子断层扫描 三维重构 超微结构 

分 类 号：Q942[生物学—植物学] Q336