智利江蓠对铜离子胁迫的生理响应  

Physiological responses of Gracilaria chilensis to copper stress

作　　者：黄中坚 孙作登 钟志海 HUANG Zhongjian;SUN zuodeng;ZHONG Zhihai(Guangxi Academy of Oceanography,Nanning 530000,P.R.China;Fisheries Development and Resources Conservation Genter,Yantai 264010,P.R.China;Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China)

机构地区：[1]广西壮族自治区海洋研究院,广西南宁530000 [2]山东省渔业发展和资源养护总站,山东烟台264010 [3]中国科学院烟台海岸带研究所,山东烟台264003

出　　处：《海洋湖沼通报》2023年第2期80-86,共7页Transactions of Oceanology and Limnology

基　　金：国家自然科学基金项目(42206131)。

摘　　要：重金属铜离子是一种重要的环境污染物,其毒性大、分布广。本文以智利江蓠(Gracilaria chilensis)为实验材料,研究了智利江蓠在不同铜离子浓度(0、25、50、100、250和500μg/L)胁迫下的生长、光合作用和生化组分,以揭示智利江蓠对铜胁迫的生理响应机制。结果表明,铜离子明显抑制了智利江蓠的生长,并随着浓度的增大抑制作用逐渐加强,当浓度达到500μg/L时,生长速率已为负值;光合作用能力、可溶性蛋白含量随着铜离子浓度的增加也明显下降,但是叶绿素a和类胡萝卜素的含量却在铜离子的胁迫下有所积累;丙二醛(MDA)含量在铜离子的胁迫下显著上升(P<0.05)。说明藻体受到了铜的损伤并随着铜离子浓度的增加而加强。Heavy metal copper is an important pollutant and copper is high toxicity and wide-spread.The effects of copper stress on the growth,photosynthesis and chemical constituents of Gracilaria chilensis were studied indoor at different copper concentrations of 0,25,50,100,250 and 500μg/L,hoping to find the physiological responses of Gracilaria chilensis to copper stress.The results showed that copper stress inhibited the relative growth rate(RGR),the inhibition increased with increasing levels of copper concentration,and the RGR become negative when copper concentration increased to 500μg/L.The photosynthetic rate and soluble protein contents significantly decreased with increasing levels of copper concentration,but the contents of chlorophyll a and carotenoid increased.The markedly increasing contents of MDA under copper stress(P<o.05),illustrating Gracilaria chilensis suffered serious damage from copper stress,and the damage became more serious with increasing levels of copper concentration.

关 键 词：智利江蓠 铜 光合作用 相对生长速率 

分 类 号：Q945.79[生物学—植物学]