反相高效液相色谱法测定消毒剂中聚六亚甲基双胍和醋酸氯己定含量  

Determination of polyhexamethylene biguanide and chlorhexidine acetate in disinfectants by reversed-phase high performance liquid chromatography

作　　者：张素英 王可洲 周东顺 ZHANG Su-ying;WANG Ke-zhou;ZHOU Dong-shun(Shandong First Medical University&Shandong Academy of Medical Sciences,Ji'nan Shangdong 250117,China)

机构地区：[1]山东第一医科大学山东省医学科学院,山东济南250117

出　　处：《中国消毒学杂志》2023年第4期254-256,共3页Chinese Journal of Disinfection

摘　　要：目的 建立一种同时测定复方消毒剂中聚六亚甲基双胍(PHMB)和醋酸氯己定(CHA)含量的高效液相色谱方法。方法 采用氰基色谱柱反相高效液相色谱法,通过梯度洗脱,对复方消毒剂中PHMB和CHA进行测定。结果 本方法能将不同聚合度的PHMB合并为一个峰,并与CHA分离,在2.0~10.0 mg/L范围内峰面积与质量浓度的线性关系良好(r>0.999);定量限分别为1.0和0.5 mg/L,检出限分别为0.3和0.15 mg/L;PHMB加标回收率为99.6%~107.8%,CHA加标回收率为95.6%~104.8%。结论 本研究建立的测定复方消毒剂中PHMB和CHA含量的反相高效液相色谱法操作简单、准确可靠,具有实际应用价值。Objective To establish a high performance liquid chromatography(HPLC)method for simultaneous determination of polyhexamethylene biguanide(PHMB)and chlorhexidine acetate(CHA)in compound disinfectants.Methods PHMB and CHA in compound disinfectant were determined by reversed-phase HPLC with cyano column and gradient elution.Results PHMB with different degrees of polymerization could be combined into one peak and separated from CHA,and the linear relationship between peak area and mass concentration was good in the range of 2.0-10.0 mg/L(r>0.999);the quantitation limits were 1.0 mg/L and 0.5 mg/L,and the detection limits were 0.3 mg/L and 0.15 mg/L,respectively;the standard recovery rates of PHMB and CHA were 99.6%-107.8%and 95.6%-104.8%,respectively.Conclusion The reversed-phase HPLC method established in this study for the determination of PHMB and CHA in compound disinfectants is simple to operate,accurate and reliable,and has practical application value.

关 键 词：聚六亚甲基双胍 醋酸氯己定 高效液相色谱 含量 检测 

分 类 号：R115[医药卫生—公共卫生与预防医学]