番茄潜叶蛾马铃薯种群幼虫肠道细菌组成结构与多样性及功能预测  

Composition,diversity and function prediction of intestinal bac⁃teria of Tuta absoluta(Meyrick)larvae in potato population

作　　者：常吕恕 杨韵 王文倩 孙淦琳 杨光源 杨航 杨进波 杜广祖[1] 陈斌[1] 张立敏[1] CHANG Lü-shu;YANG Yun;WANGWen-qian;SUN Gan-lin;YANG Guang-yuan;YANG Hang;YANG Jin-bo;DU Guang-zu;CHEN Bin;ZHANG Li-min(College of Plant Protection,Yunnan Agricultural University/State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan,Kunming,Yunnan 650201,China;Yunnan Green Biotechnology Science and Technology Co.,Ltd.,Dali,Yunnan 675600,China)

机构地区：[1]云南农业大学植物保护学院/云南生物资源保护与利用国家重点实验室,云南昆明650201 [2]云南格瑞生物科技有限公司,云南大理675600

出　　处：《南方农业学报》2023年第1期139-149,共11页Journal of Southern Agriculture

基　　金：国家自然科学基金项目(32060616);云南省大理州专家工作站项目(大科联发2020[9]号)。

摘　　要：【目的】探究番茄潜叶蛾马铃薯种群幼虫肠道细菌组成结构与多样性及功能,为番茄潜叶蛾寄主适应性机制研究及综合防治提供理论依据。【方法】采用传统分离培养法和16S rDNA序列分析对取食马铃薯的番茄潜叶蛾肠道可培养细菌进行分离鉴定;采用单分子实时测序技术对其肠道细菌16S rDNA序列进行全长测序,分析肠道细菌的组成结构与多样性,并进行功能预测分析。【结果】番茄潜叶蛾马铃薯种群幼虫肠道细菌有5门13科13属15种,优势门、科、属、种分别为厚壁菌门(Firmicutes)、肠球菌科(Enterococcaceae)、肠球菌属(Enterococcus)和蒙氏肠球菌(E.mundtii)。其中通过传统分离培养法共分离到可培养细菌2门5科5属6种,优势门、科、属、种分别是厚壁菌门(90.38%)、肠球菌科(83.71%)、肠球菌属(83.71%)和蒙氏肠球菌(83.71%),桑肠杆菌(Enterobacter mori)(6.56%)、松鼠葡萄球菌(Staphylococcus sciuri)(5.98%)和粘质沙雷氏菌(Serratia marcescens)(2.30%)为细菌主要种类。通过单分子实时测序技术在番茄潜叶蛾肠道样品中共注释到5门6纲9目10科10属10种细菌,优势种是蒙氏肠球菌(70.71%),阴沟肠杆菌(E.cloacae)(22.16%)为细菌主要种类。番茄潜叶蛾肠道细菌ACE、Shannon、Simpson和Chao1指数分别为7.22±1.91、0.69±0.43、0.28±0.19和6.33±0.94。通过KEGG代谢途径分析,发现番茄潜叶蛾马铃薯种群的肠道细菌主要参与碳水化合物、氨基酸、核苷酸和能量等的代谢,优势种蒙氏肠球菌在碳水化合物代谢和膜运输中的作用最强。【结论】番茄潜叶蛾马铃薯种群幼虫肠道细菌种类较丰富,优势种为蒙氏肠球菌,其肠道细菌可能参与碳水化合物、氨基酸和核苷酸等代谢。【Objective】To study composition,diversity and function prediction of intestinal bacteria of Tuta absoluta(Meyrick)larvae in potato population,and to provide a theoretical basis for study on host adaptation mechanism and comprehensive prevention and control of T.absoluta.【Method】Culturable bacteria in T.absoluta intestine were isolated and identified by traditional isolation and culture method and 16S rDNA sequence analysis;full length of 16S rDNA sequences of intestinal bacteria were analyzed by single molecule real-time sequencing technique,and composition structure and diversity of intestinal bacteria were analyzed,as well as function prediction.【Result】There were 5 phyla,13 families,13 genera and 15 species of bacteria in T.absoluta larvae intestine of potato population,and the dominant phylum,family,genus and species were Firmicutes,Enterococcaceae,Enterococcus and E.mundtii.There were 2 phyla,5 families,5 genera and 6 species of culturable bacteria were isolated through traditional isolation.The dominant phylum,family,genus and species were Firmicutes(90.38%),Enterococcaceae(83.71%),Enterococcus(83.71%)and E.mundtii(83.71%).Enterobacter mori(6.56%),Staphylococcus sciuri(5.98%)and Serratia marcescens(2.30%)were the main bacterial species.A total of 5 phyla,6 classes,9 orders,10 families,10 genera and 10 species were annotated by single molecule real-time sequencing technique,and E.mundtii(70.71%)was the dominant species,and E.cloacae(22.16%)was the main bacterial species.ACE,Shannon,Simpson and Chao1 indexes were 7.22±1.91,0.69±0.43,0.28±0.19 and 6.33±0.94,respectively.Based on the KEGG metabolic pathway analysis,the bacteria from T.absoluta intestine were mainly involved in metabolism pathways of carbohydrate,amino acid,nucleotide and energy.The dominant species E.mundtii was the most important in carbohydrate metabolism and membrane transport.【Conclusion】Bacteria in the intestine of T.absoluta larvae in potato population are abundant,and the dominant species is E.mundtii.These bacteria m

关 键 词：番茄潜叶蛾 马铃薯种群 可培养细菌 单分子实时测序技术 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]