黄瓜光敏色素互作因子的鉴定及表达分析  

Identification and expression analysis of phytochromeinteracting factors in cucumber

作　　者：赵金栋 廖伟民 朱楚霞 刘建萍 杨有新[2] 周勇 ZHAO Jin-dong;LIAO Wei-min;ZHU Chu-xia;LIU Jian-ping;YANG You-xin;ZHOU Yong(College of Bioscience and Bioengineering,Jiangxi Agricultural University,Nanchang,Jiangxi 330045,China;College of Agronomy,Jiangxi Agricultural University,Nanchang,Jiangxi 330045,China)

机构地区：[1]江西农业大学生物科学与工程学院,江西南昌330045 [2]江西农业大学农学院,江西南昌330045

出　　处：《南方农业学报》2023年第1期169-178,共10页Journal of Southern Agriculture

基　　金：江西省自然科学基金面上项目(20202BABL205002);江西省主要学科学术和技术带头人培养计划青年人才项目(20204BCJL23044)。

摘　　要：【目的】对黄瓜光敏色素互作因子(Phytochrome-interacting factor,PIF)家族基因进行系统分析和表达特征分析,为探究其在黄瓜生长发育与胁迫响应中的作用机理提供参考。【方法】基于黄瓜全基因组数据,通过生物信息学方法鉴定黄瓜PIF家族基因,并对家族基因的外显子—内含子结构、染色体定位、基因复制事件、系统进化树、保守基序及组织和胁迫相关表达模式等进行系统分析。【结果】从黄瓜基因组中鉴定出6个PIF基因,其不均等地分布于黄瓜的6条染色体上。基因复制事件分析结果表明,黄瓜PIF基因存在一个片段重复事件(CsPIF1a和CsPIF1b)。系统发育分析表明,来自黄瓜、拟南芥、辣椒、玉米和水稻的PIF蛋白可分为4个不同的组(PIF1、PIF2/3/6、PIF4/5、PIF7/8)。保守基序分析揭示了许多保守基序的存在,与PIF蛋白的分类一致。基因结构分析表明,CsPIF基因有5或6个内含子,与其他植物相比,内含子数量相对稳定。CsPIF基因在黄瓜各组织均有一定的表达,但在不同组织中表达量差异明显,CsPIF1a、CsPIF1b和CsPIF7分别在雌花、叶和卷须中表达量最高,CsPIF4在茎和叶中表达量明显高于其他组织,CsPIF8在茎、雌花和卷须中均具有较高的表达量,而CsPIF3在各组织中表达量相对平均。一些CsPIF基因的表达量在高温胁迫下明显下降,而在盐胁迫下明显上升。大部分CsPIF基因的表达量在霜霉病菌侵染下发生了明显的变化,其中CsPIF4和CsPIF8的表达量在黄瓜接种根结线虫后均有明显上升。【结论】一些黄瓜PIF基因的表达量在不同非生物和生物胁迫下发生明显变化,推测其在黄瓜逆境响应过程中发挥着重要的调控作用。【Objective】Identification and expression analysis of cucumber phytochrome-interacting factor(PIF)family genes would lay a foundation for further exploration to clarify their functions in cucumber growth,development,and biotic and abiotic stress response.【Method】Cucumber PIF family genes were identified at the whole cucumber genome level by bioinformatics methods,and the exon-intron structure,chromosome location,gene duplication,phylogenetic relationships,conserved motifs,tissue and stress-associated expression profiles were also analyzed.【Result】A total of 6 PIF family genes were identified in cucumber genome,which were unevenly distributed on six chromosomes.Gene duplication analysis showed that these cucumber PIF genes made up a segmental duplication event(CsPIF1a and CsPIF1b).Phylogenetic analysis showed that PIF proteins from cucumber,Arabidopsis,pepper,maize and rice could be divided into four different groups(PIF1,PIF2/3/6,PIF4/5,and PIF7/8).Conservative motif analysis revealed the existence of many conserved motifs,which was consistent with the classification of PIF proteins.Gene structure analysis showed that CsPIF genes had 5 or 6 introns,which was relatively stable when compared to other plants.CsPIF genes expressed in all tissues of cucumber,and its expression were greatly different in various tissues.For example,CsPIF1a,CsPIF1b,and CsPIF7 showed the highest expression of transcripts infemale flower,leaf,and tendril,respectively,CsPIF4 displayed higher expression levels in stem and leaf than in other tissues,CsPIF8 was highly expressed in stem,female flower,and tendril,while CsPIF3 showed constitutive expression in different tissues.In addition,some of them were down-regulated and up-regulated by heat and salt stress treatments,respectively.Most CsPIF genes hadgreatly altered expression in response to downy mildew(DM)inoculation,and the expression levels of CsPIF4 and CsPIF8 were observably increased after root-knot nematode(RKN)treatment.【Conclusion】The expression levels of some CsPIF

关 键 词：黄瓜 PIF基因家族 表达分析 非生物胁迫 生物胁迫 

分 类 号：S642.2[农业科学—蔬菜学] Q751[农业科学—园艺学]