春季栽培下花椰菜产量及品质性状与SSR标记的关联分析  

Association analysis of yield and quality traits with SSR markers in cauliflower under spring cultivation

作　　者：朱世杨[1] 刘庆[1] 钟伟杰 唐征[1] 罗天宽[1] 徐谦[1] ZHU Shi-yang;LIU Qing;ZHONGWei-jie;TANG Zheng;LUO Tian-kuan;XU Qian(Southern Zhejiang Key Laboratory of Crop Breeding,Wenzhou Vocational College of Science and Technology,Wenzhou,Zhejiang 325006,China)

机构地区：[1]温州科技职业学院浙南作物育种重点实验室,浙江温州325006

出　　处：《南方农业学报》2023年第1期179-189,共11页Journal of Southern Agriculture

基　　金：浙江省农业(蔬菜)新品种选育重大科技专项(2021C02065);温州市科技计划项目(Z20170006,K20200007,X20210086)。

摘　　要：【目的】通过关联分析挖掘与春季栽培下花椰菜花球产量及品质性状关联的分子标记,为春季生态型花椰菜花球高产、优质标记辅助选择育种提供有益的参考。【方法】用多态性较好的26对SSR标记对80份花椰菜自交系进行群体结构分析,并采用Tassel 2.1的一般线性模型(GLM)对该群体2018和2019年春季花球的3个产量性状(花球重、花球纵径和横径)及4个品质性状(维生素C、可溶性糖、叶绿素及类胡萝卜素含量)与SSR标记进行关联分析。【结果】80份自交系的花球重、花球纵径和横径及维生素C、可溶性糖、叶绿素类和胡萝卜素含量的变异系数2年平均值分别为60.0%、20.7%、23.9%、27.3%、27.6%、64.1%和61.6%,表明80份自交系的花球产量及品质性状变异丰富。邻接法系统聚类和群体结构分析均可将80份自交系分为3个类群,不同分类结果与品种的地域来源、花球紧实度间均无直接关系。基于GLM关联分析,2年试验均能重复检测出与花球产量、品质性状显著相关的标记共6个(P<0.05),对各性状变异解释率变幅为4.32%~13.96%。其中标记Ol10F07和BoGMS1042与花球重关联,解释率分别为7.71%和13.96%;标记Na14H11与花球纵径关联,解释率为5.56%;标记Na10D07、Na14H11、BoGMS1042和BoDCTD1与花球横径关联,标记BoDCTD1解释率最高,为12.89%;标记Ra2F11、Na10D07和Na14H11与维生素C含量关联,Ra2F11解释率最高,为11.12%;标记Ra2F11均与可溶性糖、叶绿素和类胡萝卜素含量关联,解释率分别为11.25%、11.73%和8.28%。【结论】春季栽培下80份花椰菜自交系产量及品质相关性状的遗传变异丰富,检测到6个SSR标记与花球产量品质性状显著关联,且存在同一标记与多个性状关联或同一个性状与多个标记关联的现象,为春季生态型花椰菜高产优质育种提供了参考分子标记。【Objective】The molecular markers related with curd yield and quality traits was explored by association analysis,which could provide candidate markers for utilization in marker-assisted breeding(MAS)of cauliflower in spring with high yield and high quality characters.【Method】Twenty-six pairs of simple sequence repeat(SSR)markers were used to unlock the population structure of 80 cauliflower inbred lines.The association analysis using the general linear model(GLM)in TASSEL 2.1 software was adopted to identify the SSR markers associated with three yield traits(curd weight,lengthways diameter and transverse diameter of curd)and four quality traits(contents of vitamin C,soluble sugar,chlorophyll and carotenoid)in curd of this population in spring 2018 and spring 2019.【Result】The results showed that two-year average of coefficient of variation(CV)of curd weight,lengthways diameter of curd,transverse diameter of curd,vitamin C,soluble sugar,chlorophyll and carotenoid of the inbred lines were 60.0%,20.7%,23.9%,27.3%,27.6%,64.1%and 61.6%,respectively.It showed that the 80 inbred lines had rich genetic variation in curd yield and quality related traits.On basis of SSR marker data,the 80 cauliflower inbred lines were both classified into three categories by Neighborjoining analysis and population structure analysis respectively.The classification patterns of most cauliflower inbred lines were not consistent with their curd solidity or geographic origins.Further based on GLM analysis,a total of 6 SSR markers were found to be significantly associated with yield and quality traits in two years(P<0.05),whose explained variance ranged from 4.32%to 13.96%.Among them,the markers of Ol10F07 and BoGMS1042 were associated with curd weight,and the explanation rates were 7.71%and 13.96%respectively.Marker Na14H11 was associated with lengthways diameter of curd,and the explanation rate was 5.56%.Markers Na10D07,Na14H11,BoGMS1042 and BoDCTD1 were associated with transverse diameter of curd,of which BoDCTD1 had the highest ex

关 键 词：花椰菜 产量品质性状 SSR标记 群体结构 关联分析 

分 类 号：S635.3[农业科学—蔬菜学]