不同光照处理对银柴胡愈伤组织形成及褐化的影响  

Effect of different light treatments on callus formation and browning of Stellaria dichotoma

作　　者：王煜纬 代晓华[1] Wang Yuwei;Dai Xiaohua(School of Agriculture,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏大学农学院,宁夏银川750021

出　　处：《农业科学研究》2023年第1期34-37,共4页Journal of Agricultural Sciences

基　　金：宁夏重点研发项目“中部干旱带特色作物节水增效综合生产技术研究与集成示范”(2019BBF02029)。

摘　　要：为建立银柴胡高效快繁体系,保证银柴胡资源的可持续利用,采用组培技术,以当年生银柴胡植株腋芽为外植体,以MS+NAA(4 mg/L)+6-BA(1 mg/L)为愈伤组织诱导培养基,研究不同暗处理时间与光照强度对愈伤组织形成及褐化的影响。结果表明:影响愈伤组织的主要因素为暗处理时间,随着暗处理时间增加,银柴胡出愈率显著提高;影响愈伤组织褐化的因素为光照强度和暗处理时间,在8000、10000及16700 lx光照下,随着光照强度增强,银柴胡愈伤组织褐化率升高。To establish an efiicient and fast breeding system of Stellaria dichotoma and ensure the sustainable development and utilization of resources from the medicinal plant Stellaria dichotoma,using tissue culture techniques.The effect of different dark treatment time and light intensity on the callus formation and browning was investigated by using MS+NAA(4 mg/L)+6-BA(1 mg/L)as the induction medium and conducting a histoculture experiment on the axillary buds of current-growth Stellaria dichotoma.The results show that the dark treatment time mainly affects the callus production,and the callus induction increased significantly with the increase of dark treatment time.Both light intensity and dark treatment time aHiect the browning,and the browning rate increases with the increase of light intensity at 8000,10000 and 16700 lx.

关 键 词：银柴胡 暗处理时间 光照强度 愈伤组织 

分 类 号：S567.2[农业科学—中草药栽培]