氧化还原伴侣工程:P450低效问题的解决方案之一  

Redox Partner Engineering:A Solution to the Low Catalytic Efficiency of P450s

作　　者：张岩峰 叶丽丹[1] 于洪巍[1] ZHANG Yan-feng;YE Li-dan;YU Hong-wei(College of Chemical and Biological Engineering,Zhejiang University,Hangzhou 310027)

机构地区：[1]浙江大学化学工程与生物工程学院,杭州310027

出　　处：《生物技术通报》2023年第4期10-23,共14页Biotechnology Bulletin

基　　金：国家重点研发计划(2020YFA0908400)。

摘　　要：细胞色素P450酶(CYPs或P450s)可将O_(2)的一个原子插入有机底物同时将另一个原子还原为水,广泛参与各种合成代谢和分解代谢过程,所以一直以来都是生物技术领域关注的焦点。在催化循环底物的氧化依赖于氧化还原伴侣向血红素铁传递电子,因此电子转移是P450s催化过程中的限速步骤。利用不同方法优化蛋白质-蛋白质相互作用以提高P450系统的电子转移效率,被称为“氧化还原伴侣工程”,是目前工程化P450s的重要手段之一,并取得了卓有成效的进展。本文将着重介绍关于氧化还原伴侣组分替换组装、P450酶与氧化还原伴侣融合及P450酶与氧化还原伴侣作用界面修饰等方面的进展,期望为未来该方面的工作提供一定的指导作用。Cytochrome P450 enzymes(CYPs or P450s)can insert one atom of O2 into organic substrates and reduce another atom to water.As P450s are widely involved in various anabolic and catabolic processes,they have always been a focus of biotechnology.Oxidation of substrates in the catalytic cycle relies on the transfer of electrons from redox partners to the heme iron,suggesting electron transfer as a ratelimiting step in the catalytic process of P450s.Optimization of protein-protein interactions to improve the electron transfer efficiency of the P450 system,known as“redox partner engineering”,is one of the important means to engineer P450s,and has achieved fruitful progress.This paper will focus on the progress in the replacement and assembly of redox partners,fusion of P450 enzymes with redox partners,and interface modification between P450 enzymes and redox partners,aiming to provide some guidance for future work in this area.

关 键 词：细胞色素P450酶 氧化还原伴侣 电子转移 组件替换 融合酶 

分 类 号：Q814[生物学—生物工程]