猕猴桃过氧化氢酶基因家族全基因组鉴定与表达分析  

Genome-wide Identification of Catalase Family Genes and Expression Analysis in Kiwifruit

作　　者：赖瑞联[1] 冯新[1] 高敏霞[1] 路喻丹 刘晓驰 吴如健[1] 陈义挺[1] LAI Rui-lian;FENG Xin;GAO Min-xia;LU Yu-dan;LIU Xiao-chi;WU Ru-jian;CHEN Yi-ting(Fruit Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350013)

机构地区：[1]福建省农业科学院果树研究所,福州350013

出　　处：《生物技术通报》2023年第4期136-147,共12页Biotechnology Bulletin

基　　金：福建省人民政府与中国农业科学院农业高质量发展超越“5511”协同创新工程(KXXYJBG2021006);福建省种业创新与产业化工程(zycxny2021010);福建省省属公益类科研院所基本科研专项(2019R1028-5);福建省农业科学院科技创新团队(CXTD2021009-2)。

摘　　要：过氧化氢酶(Catalase,CAT)是植物主要的抗氧化酶之一。为揭示猕猴桃CAT(AcCAT)基因家族序列特征和潜在功能,对其进行了全基因组鉴定和表达分析。对AcCAT基因家族进行全基因组鉴定和生物信息学分析,并研究其在不同器官以及果实贮藏过程中的表达变化。从猕猴桃基因组中鉴定出9个AcCAT基因并根据其在染色体上的位置分别命名为AcCAT1-AcCAT9,不同成员之间蛋白理化性质、基因结构、保守基序、启动子作用元件等存在较高相似性。AcCAT基因不均匀地分布在猕猴桃的4条染色体上,其中的5个AcCAT基因形成2个串联基因簇,6个AcCAT基因发生片段复制。基于psRNAtarget分析,AcCAT基因可能主要受miR166家族调控。系统进化分析结果显示,来源于猕猴桃、茶树、棉花和水稻的23个CAT蛋白分为3个类群,其归类并未完全按物种划分。在不同猕猴桃器官中,AcCAT3主要在根中表达,AcCAT1和AcCAT4主要在花中表达,AcCAT2、AcCAT5、AcCAT6、AcCAT8和AcCAT9主要在叶中表达,AcCAT7则同时在根和花中高水平表达;在猕猴桃果实贮藏过程中,AcCAT5和AcCAT6表达水平逐渐降低,AcCAT1和AcCAT2主要在贮藏前期表达,而AcCAT3、AcCAT4和AcCAT8主要在贮藏后期表达。此外,miR166家族成员在果实贮藏过程中的表达量逐步升高。AcCAT基因在进化过程中存在保守性,其在猕猴桃生长发育和果实贮藏软化过程中发挥调控作用。Catalase(CAT)is one of the main antioxidant enzymes in plants.In order to reveal the sequence characteristics and expression patterns of kiwifruit CAT family genes(AcCATs),genome-wide identification and expression analysis were performed.The genomewide identification and bioinformatics analysis of AcCAT family genes were carried out,and its expression changes in different organs and during fruit storage were analyzed.Nine genes of AcCAT family were identified from kiwifruit genome using bioinformatics method and named as AcCAT1-AcCAT9 respectively according to their chromosome location information.There were high similarities in protein physicochemical properties,gene structure,conserved motifs and cis-acting elements in different members.AcCAT genes were unevenly distributed on four chromosomes,five AcCAT genes formed two tandem duplication gene clusters,and six AcCAT genes had segmental duplication.By psRNAtarget analysis,AcCAT genes were predicted to be mainly regulated by miR166 family members.Phylogenetic analysis showed that 23 CAT proteins from kiwifruit,Camellia sinensis,Gossypium hirsutum and Oryza sativa could be classified into three groups,and the classification was not completely conducted by species.Transcriptome analysis of different kiwifruit organs revealed that AcCAT3 expressed highly in the root,AcCAT1 and AcCAT4 expressed highly in the flower,AcCAT2,AcCAT5,AcCAT6,AcCAT8 and AcCAT9 were mainly expressed in the leaf,while AcCAT7 was up-expressed both in the root and flower.During the storage of kiwifruit,the expression levels of AcCAT5 and AcCAT6 decreased gradually,but AcCAT1 and AcCAT2 were up-expressed in early stage,while the expression of AcCAT3,AcCAT4 and AcCAT8 increased in late stage.Additionally,the expressions of miR166 family members increased during this process.AcCAT family genes are conservative in evolution,and they play important regulatory roles in the growth and during the storage of kiwifruit.

关 键 词：猕猴桃 过氧化氢酶 全基因组鉴定 基因表达 

分 类 号：S663.4[农业科学—果树学]