SPE-HPLC双检测器法同时测定地表水中的苯并[a]芘和阿特拉津含量及不确定度评定  

Simultaneous determination of Benzo[a]pyrene and Atrazine in surface water by SPE-HPLC dual detector and evaluation of uncertainty

作　　者：褚琳琳 吕晓昊 耿冬梅 张然 孔翠羽 CHU Linlin;LYU Xiaohao;GENG Dongmei;ZHANG Ran;KONG Cuiyu(Shandong Weihai Eco-Environment Monitoring Center,Weihai Shandong 264200)

机构地区：[1]山东省威海生态环境监测中心,山东威海264200

出　　处：《首都师范大学学报（自然科学版）》2023年第2期35-42,共8页Journal of Capital Normal University：Natural Science Edition

基　　金：江西省教育厅科学技术研究项目青年项目(GJJ190613);山东省高等学校优秀青年创新团队项目(2019KJM008)。

摘　　要：采用全自动固相萃取(SPE)-高效液相色谱(HPLC)双检测器法同时定量分析地表水中的苯并[a]芘(BaP)和阿特拉津(AT)含量,建立了BaP和AT含量不确定度的分析方法。从样品前处理过程、样品重复测定、标准溶液配制和标准曲线拟合等方面评定了不确定度分量的贡献。评定结果显示:在影响检测结果的4个分量中,HPLC测定地表水中BaP含量时,标准曲线拟合和样品前处理过程引入的不确定度对结果影响较大,当BaP质量浓度为2.01 ng/L时,其扩展不确定度(U)为0.24 ng/L(k=2);测定AT含量时,标准溶液配制和样品重复测定引入的不确定度对结果影响较大,当AT质量浓度为50.09 ng/L时,U=4.72 ng/L(k=2)。A method for the determination of Benzo[a]pyrene and Atrazine in surface water by automatic solid phase extraction and high-performance liquid chromatography with double detector was developed.The contribution of uncertainty component was evaluated from four aspects:sample pretreatment process,sample repeatability determination,standard solution preparation and standard curve fitting.Among the four uncertainty components,the uncertainty introduced by standard curve fitting and sample pretreatment process has a great influence on the determination of Benzo[a]pyrene content.When the content of Benzo[a]pyrene in surface water was 2.01 ng/L,the extended uncertainty was 0.24 ng/L(k=2).In the determination of Atrazine content,the uncertainty introduced by standard solution preparation and sample repeatability determination has great influence on the results.When Atrazine content was 50.09 ng/L,the extended uncertainty was 4.72 ng/L(k=2).

关 键 词：不确定度评定 苯并[A]芘 阿特拉津 固相萃取 高效液相色谱法 

分 类 号：O657.7[理学—分析化学] P207[理学—化学]