红锥根系响应石墨烯的转录组学研究  

Influence of graphene on different pathways of Castanopsis hystrix roots based on transcriptome analysis

作　　者：白秀丽 庞敏 刘泽慧 乔俊[1] 李伟佳 淮泽勋 王海雁[1] 赵建国[1] 陈志文 BAI Xiuli;PANG Min;LIU Zehui;QIAO Jun;LI Weijia;HUAI Zexun;WANG Haiyan;ZHAO Jianguo;CHEN Zhiwen(Institute of Carbon Materials Science,College of Chemistry and Chemical Engineering,Shanxi Datong University,Datong Shanxi 037009)

机构地区：[1]山西大同大学炭材料研究所,化学与工程学院,山西大同037009

出　　处：《首都师范大学学报（自然科学版）》2023年第2期43-52,共10页Journal of Capital Normal University：Natural Science Edition

基　　金：国家自然科学基金面上项目(52071192);2018年山西省科技成果转化引导专项项目(201804D131041);山西大同大学博士科研启动基金项目(2019-B-03);山西大同大学2021年研究生教育创新项目(21CX10)。

摘　　要：为了研究石墨烯对植物根系响应的分子机制和转录组学,本文以0、25、33和50 mg/L共4组质量浓度的石墨烯溶液培育红锥(Castanopsis hystrix Miq.)幼苗,其中石墨烯溶液质量浓度为0指以蒸馏水培育,并对红锥幼苗的根系发育情况、基因表达量的变化、差异表达基因的富集通路和范围进行了分析。与0相比,25 mg/L石墨烯溶液对红锥幼苗根系发育的促进效果最佳(t=2.86~7.80,P<0.05);转录组数据表明,25 mg/L石墨烯溶液处理红锥根系后,导致12845个基因的表达量发生差异,其中石墨烯诱导10899个基因表达量上调,抑制1946个基因的表达量。通过京都基因与基因组百科全书(KEGG)通路富集性分析表明:表达量上调的基因主要富集于126个KEGG通路,表达量下调的基因富集于99个KEGG通路;另外,110个转录因子基因差异表达,可归类于33个不同家族,其中ERF、WRKY、MYB、NAC、bHLH、TCP和Trihelix共7个家族基因与红锥根系发育有关;9种植物激素信号转导通路相关的差异表达基因结果表明生长素、细胞分裂素、赤霉素、乙烯、水杨酸、油菜素内酯和独脚金内酯共7种激素相关基因表达量的改变与红锥根系生长密切有关。In order to investigate the molecular mechanism and transcriptomics of the response of graphene to the roots of Castanopsis hystrix Miq plant.Seedlings were cultivated with 4 groups of graphene solution concentrations of 0,25,33 and 50 mg/L,0 refers to distilled water culture,the root development,gene expression,enrichment pathway and range of differentially expressed genes were analyzed.Compared with 0 group,25 mg/L graphene solution had the best promoting effect on root development of Castanopsis hystrix seedlings(t=2.86 to 7.80,P<0.05).Transcriptomic data showed that the expression levels of 12845 genes were changed after 25 mg/L graphene solution was applied to Castanopsis hystrix roots,among which 10899 genes were up-regulated and 1946 genes were inhibited.According to the Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis,the up-regulated genes were mainly enriched in 126 KEGG pathways,and down-regulated genes were enriched in 99 KEGG pathways.In addition,110 transcription factor genes were differentially expressed and could be classified into 33 different families.Among them,7 transcription factor family genes,including ERF,WRKY,MYB,NAC,bHLH,TCP and Trihelix were related to root development of Castanopsis hystrix.Analyses of differentially expressed genes related to plant hormone signal transduction pathways showed changes in the expression levels of 7 hormone-related genes,including auxin,cytokinin,gibberellin,ethylene,salicylic acid,brassinosteroid and strigolactone,were closely related to the root growth of Castanopsis hystrix.

关 键 词：石墨烯 红锥 根系 差异表达基因 转录因子与激素 

分 类 号：O613[理学—无机化学] Q942[理学—化学]