A finTRIM member 100 (FTR100) is unique to Otomorpha fish for constitutive regulation of IFN response  

作　　者：Wen-Hao Guo Cheng Dan Xiu-Ying Gong Yi-Lin Li Hao-Yu Sun Zi-Ling Qu Li-Li An Xiang Zhao Jie Mei Jian-Fang Gui Yi-Bing Zhang 

机构地区：[1]State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,430072,China [2]University of Chinese Academy of Sciences,Beijing,10049,China [3]College of Fisheries,Huazhong Agricultural University,Wuhan,430070,China [4]The Innovation Academy of Seed Design,Chinese Academy of Sciences,Wuhan,430072,China

出　　处：《Water Biology and Security》2023年第2期80-90,共11页水生生物与安全（英文）

基　　金：supported by grants from the National Key R&D Program of China(2022YFF1000302);the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA24010308);the National Natural Science Foundation(31972826 and 32102838);the Freshwater Ecology and Biotechnology Laboratory(2019FBZ04).

摘　　要：Vertebrate interferon(IFN)expression is fine-tuned in order to avoid excessive tissue injury under normal conditions and during virus infection.FinTRIM(fish novel TRIM,FTR)proteins are reported to regulate the fish IFN response.Here,we identify a novel finTRIM gene from yellow catfish(Pelteobagrus fulvidraco),which is sequentially named PfFTR100 according to the nomenclature rule in zebrafish.Genome-wide analyses reveal that FTR100 is unique to Otomorpha fish,with a single copy in spite of additional genome duplication in some fish species.Considering that few of the 99 finTRIM genes identified in zebrafish are conserved in main fish branches and most,such as FTR100,are unique to distinct branches due to lineage-specific expansion of finTRIM genes,we develop a nomenclature for newly cloned finTRIM genes from different fish species.PfFTR100 mRNA is not induced by virus infection,with a relatively high expression level comparable to that of cellular IFN and some IFN-stimulated genes(ISGs)in virally-infected tissues.However,ectopically-expressed PfFTR100 protein is attenuated in virally-infected cells through the proteasomal-dependent pathway.Overexpression of PfFTR100 promotes SVCV replication by downregulating the constitutive and inducible IFN response via a mechanism by which PfFTR100 targets IRF3 and IRF7 to attenuate their mRNA levels rather than their protein levels.Our results indicate that yellow catfish FTR100 is essential for homeostatic regulation of fish tonic IFN response.

关 键 词：Constitutive expression FTR100 Homeostatic regulation Lineage-specific expansion Tonic IFN response 

分 类 号：Q95[生物学—动物学]