亚硝态氮慢性胁迫对斑节对虾(非洲群体)行为、消化酶活力和非特异性免疫的影响  

Effects of Chronic Nitrite Stress on Behavior,Digestive Enzyme Activities and Non-Specific Immunity of an African Population of Penaeus monodon

作　　者：王友红 王晓璐 于晓清 许拉 盖春蕾 王淑娴 刘洪军 刁菁 叶海斌 樊英 付壤辉 Wang Youhong;Wang Xiaolu;Yu Xiaoqing;Xu La;Gai Chunlei;Wang Shuxian;Liu Hongjun;Diao Jing;Ye Haibin;Fan Ying;Fu Ranghui(Marine Science Research Institute of Shandong Province,Key Laboratory for Disease Control in Mariculture of Shandong Province,Qingdao Municipal Engineering Research Center of Aquatic Biological Quality Evaluation and Application,Qingdao 266104,China;Shandong Youfa Aquaculture Company Limited,Binzhou 251900,China)

机构地区：[1]山东省海洋科学研究院山东省海水养殖病害防治重点实验室,青岛市水产生物品质与利用工程研究中心,山东青岛266104 [2]山东省友发水产有限公司,山东滨州251900

出　　处：《中国海洋大学学报（自然科学版）》2023年第6期43-49,共7页Periodical of Ocean University of China

基　　金：山东省虾蟹产业技术体系项目(SDAIT-13);国家虾蟹产业技术体系项目(CARS-48)资助。

摘　　要：为探讨亚硝态氮(NO_(2)-N)慢性胁迫对斑节对虾(Penaeus monodon)(非洲群体,以下称金刚虾)生理特性的影响,本实验以体质量为(6.06±0.76)g的金刚虾为研究对象,测定不同NO_(2)-N浓度下金刚虾的行为、非特异性免疫以及消化功能。研究显示,NO_(2)-N浓度≤6 mg/L时,金刚虾的活力、摄食不受影响;NO_(2)-N浓度≥8 mg/L时,金刚虾摄食缓慢、游动异常、胃肠道模糊;NO_(2)-N浓度为14 mg/L时,金刚虾开始死亡。金刚虾肝胰腺中脂肪酶(Lipase)、胰蛋白酶(Trypsin)活性在NO_(2)-N浓度为6 mg/L时达到最大值,之后开始下降,当NO_(2)-N浓度升至14 mg/L时活性最低,与对照组差异显著。NO_(2)-N浓度由0上升至14 mg/L,血淋巴谷胱甘肽(GSH)含量、酸性磷酸酶(ACP)活性呈“先升高后下降”的趋势,谷草转氨酶(GOT)、谷丙转氨酶(GPT)的活性则呈“先升高后下降再升高”的趋势,NO_(2)-N浓度为6 mg/L时,GHS、ACP活性达到最大值;NO_(2)-N浓度为14 mg/L时,GOT、GPT活性达到最大值,与对照组差异显著。综合以上结果,工厂化养殖金刚虾时,建议将水体中NO_(2)-N浓度控制在6 mg/L以下。In order to explore the effects of chronic nitrite(NO_(2)-N)stress on physiological characteristics of an African population of Penaeus Monodon,the behavior,non-specific immunity and digestive of the prawns were measured under different concentrations of NO_(2)-N.The experimental group and the control group contained individuals with the body weight of(6.06±0.76)g.The concentration of NO_(2)-N in the experimental group was gradually increased by 0.5 mg/L after daily water change while that in the control group was only changed without any operation.The experiment was carried out for 28 days.The results showed that when NO_(2)-N concentration was≤6 mg/L,the activity and feeding were not affected.When NO_(2)-N concentration was≥8 mg/L,the feeding ability was slow,swimming was abnormal,and the gastrointestinal tract was blurred.When NO_(2)-N concentration was 14 mg/L,prawns began to die.Lipase and trypsin in shrimp hepatopancreas increased to the maximum at 6 mg/L of NO_(2)-N,then began to decline,and the lowest activity was observed at 14 mg/L of NO_(2)-N,which was significantly different from that of control.When the concentration of NO_(2)-N increased from 0 to 14 mg/L,the content of glutathione(GSH)and the activity of acid phosphatase(ACP)in hemolymph showed a trend,increasing first and then decreasing,while the activities of glutamic oxalacetic aminotransferase(GOT)and alanine aminotransferase(GPT)showed a trend,increasing first and then decreasing and finally increasing.When the concentration of NO_(2)-N was 6 mg/L,the activity of GHS and ACP reached the maximum.When the concentration of NO_(2)-N was 14 mg/L,the activities of GOT and GPT reached the maximum which were significantly different from those of control.Therefore,it is recommended that NO_(2)-N in water should be controlled below 6 mg/L during factory culture of P.monodon.

关 键 词：斑节对虾(非洲群体) 亚硝态氮 行为 非特异性免疫 消化酶 

分 类 号：S968.22[农业科学—水产养殖]