高海拔温度变化对活性污泥中磷去除及相关代谢途径的影响  

Effects of temperature changes at high altitude on phosphorus removal and related metabolic pathways in activated sludge

作　　者：王俊 郭明哲 尤俊豪 宗永臣 傅椿惠 张东艳 WANG Jun;GUO Ming-zhe;YOU Jun-hao;ZONG Yong-chen;FU Chun-hui;ZHANG Dong-yan(Water Conservancy Project&Civil Engineering College,Tibet Agriculture&Animal Husbandry University,Linzhi 860000,China;State Key Laboratory of Coal Combustion,Huazhong University of Science and Technology,Wuhan 430074,China)

机构地区：[1]西藏农牧学院水利土木工程学院,西藏林芝860000 [2]华中科技大学,煤燃烧国家重点实验室,湖北武汉430074

出　　处：《中国环境科学》2023年第5期2364-2372,共9页China Environmental Science

基　　金：国家自然科学基金资助项目(51868069);煤炭燃烧国家重点实验室基础项目(FSKLCCA2012)。

摘　　要：基于高原生境下A2O工艺,探讨不同温度条件下厌氧、缺氧和好氧3个反应器的除磷性能,讨论了优势聚磷酸盐累积生物(PAOs)和糖原累积生物(GAOs)以及相关代谢途径中基因对温度变化的响应情况.结果表明,在20℃时磷去除效果最佳(82.99%~88.39%),但低于平原地区同工艺研究下的除磷性能.通过分析优势微生物PAOs和GAOs群落结构发现,GAOs不具竞争优势,不随温度的升高而增强.在10℃时存在污泥膨胀和在25℃时不利于PAOs生长的环境条件下,不动杆菌属(Acinetobacter)(PAO)、脱氯单胞菌属(Dechloromonas)和氢嗜胞菌属(Hydrogenophaga)(反硝化PAOs)在缺氧反应器中相对丰度较高,对缺氧反应器中的磷去除率的提高具有一定的贡献.此外,研究确定了糖酵解(EMP)是葡萄糖代谢的主要途径.多羟基戊酸盐(PHV)的主要合成途径是Propionyl-CoA.厌氧和好氧反应器所需ATP均主要来源于三羧酸循环(TCA cycle)途径.The A^(2)O process in highland habitats was used in this paper to investigate the phosphorus removal performance of anaerobic,anoxic and aerobic reactors under different temperature conditions,including the response of dominant polyphosphate accumulating organisms(PAOs)and glycogen accumulating organisms(GAOs)and related metabolic pathways to temperature changes.The results show that the best phosphorus removal performance(82.99%~88.39%)was achieved at 20℃,but lower than that of the same process in the plains;and GAOs did not have competitive advantage and did not increase with an increase in temperature.The relative abundance of Acinetobacter(PAO),Dechloromonas and Hydrogenophaga(denitrifying PAOs)was higher in the anoxic reactor under the environmental conditions of sludge swelling at 10℃and unfavorable growth of PAOs at 25℃,which could be attributed to the improvement of phosphorus removal in the anoxic reactor.In addition,glycolysis(EMP)was identified as the main pathway of glucose metabolism.Evidently,the main synthetic pathway of polyhydroxyvalerate(PHV)was Propionyl-CoA;and the ATP required for both anaerobic and aerobic reactors came mainly from the tricarboxylic acid cycle(TCA cycle)pathway.

关 键 词：高原生境 除磷性能 优势PAOs GAOs 相关代谢途径 

分 类 号：X703.5[环境科学与工程—环境工程]