一株太空培育螺旋藻诱变株的皮肤修护和保湿研究  

Study on skin repairing and skin moisturizing of a Spirulina Platensis mutant induced by space-flight breeding

作　　者：杨登亮 周雨叶 向文洲[4] 林盛杰 李传茂 YANG Deng-liang;ZHOU Yu-ye;XIANG Wen-zhou;LIN Sheng-jie;LI Chuan-mao(Gongdong Danz Group Co.Ltd.,Guangzhou,Guangdong 510410,China;Guangzhou Keneng Cosmetics Scientific Research Co.,Ltd.,Guangzhou,Guangdong 510890,China;Guangzhou Baiyun Lianjia Fine Chemistry Factory,Guangzhou,Guangdong 510445,China;Key Laboratory of Tropical Marine Bio-resources and Ecology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou,Guangdong 510301,China)

机构地区：[1]广东丹姿集团有限公司,广东广州510410 [2]广州市科能化妆品科研有限公司,广东广州510890 [3]广州市白云联佳精细化工厂,广东广州510445 [4]中国科学院南海海洋研究所中国科学院热带海洋生物资源与生态重点实验室,广东广州510301

出　　处：《日用化学品科学》2023年第5期15-18,共4页Detergent & Cosmetics

基　　金：广东省重点领域研发计划资助(2020B1111030004)。

摘　　要：利用太空育种方法,培育得到新型的钝顶螺旋藻诱变株H11,超声法提取水溶性胞内多糖,使用斑马鱼胚胎尾鳍检测其皮肤修护能力,使用3D表皮模型检测其对天然保湿因子分泌的影响。实验结果表明,野生株和诱变株螺旋藻多糖得率分别为4.88%和20.32%(干重);野生株和诱变株螺旋藻多糖在0.1 mg/mL浓度下,斑马鱼胚胎尾鳍再生生长率分别提高1.44%和13.12%;在1%浓度下3D表皮模型反式尿刊酸的生成分别增加了16.50%和156.43%。结果表明,螺旋藻诱变株多糖含量远远高于野生株,并且表现出优于野生株螺旋藻的皮肤修护和保湿能力。With the method of space-flight breeding,a new strain of Spirulina Platensis mutant,H11,was cultivated.The water-soluble intracellular polysaccharides were extracted with ultrasonic method.The effects on skin repairing and natural moisturizing factor(NMF)secretion were measured using zebrafish embryo caudal fin and 3D human epidermis model.The results showed that the polysaccharide content of wild type and mutant Spirulina Platensis was 4.88%and 20.32%(dry weight),respectively.After the application of wild type and mutant Spirulina Platensis,the regrowth rate of zebrafish embryo caudal fin was respectively increased by 1.44%and 13.12%at the concentration of 0.1 mg/mL;the trans-urocanic acid production of 3D human epidermis model was respectively increased by 16.50%and 156.43%at the concentration of 1%.The polysaccharide content of mutant Spirulina Platensis was much higher than that of the wild strain,and meanwhile,the mutant Spirulina Platensis also showed better skin repairing and moisturizing ability.

关 键 词：螺旋藻 太空育种 多糖 皮肤模型 斑马鱼 修护 保湿 

分 类 号：TQ658[化学工程—精细化工]