CD3单克隆抗体诱导人源化细胞因子风暴小鼠模型  

CD3 Monoclonal Antibody Induces Humanized Cytokine Storm Mice Model

作　　者：刘潺潺 姚袁媛 王钟情 张立波 李婷 LIU Chanchan;YAO Yuanyuan;WANG Zhongqing;ZHANG Libo;LI Ting(Department of Neurology,University of Chinese Academy of Sciences-Shenzhen Hospital,Shenzhen 518000,China;Department of Gynaecology,University of Chinese Academy of Sciences-Shenzhen Hospital,Shenzhen 518000,China;Department of Emergency,University of Chinese Academy of Sciences-Shenzhen Hospital,Shenzhen 518000,China)

机构地区：[1]中国科学院大学深圳医院(光明)神经内科,深圳518000 [2]中国科学院大学深圳医院(光明)妇科,深圳518000 [3]中国科学院大学深圳医院(光明)急诊科,深圳518000

出　　处：《实验动物科学》2023年第2期22-24,共3页Laboratory Animal Science

基　　金：深圳光明区经济发展专项资金(2021R01125);中国博士后科学基金新冠肺炎疫情防控专项(2020T130047ZX)

摘　　要：目的构建CD3单克隆抗体诱导人源化细胞因子风暴小鼠模型。方法提取健康成人的外周血单核细胞(PBMCs),静脉注射10~12周龄NSG小鼠后,随机分为2组:(1)PBMCs组(2)OKT3(CD3单抗,anti-CD3 mAb,0.1 mg/mL)+PBMCs。实验组6 d后注射CD3抗体0.5 mg/kg,6 h后ELISA检测血清IL-10、CTLA-4、INF-γ、IL-6、IL-2、IL-7和TGF-β水平。结果相比对照组,使用OKT3能有效诱导出细胞因子风暴,其中IL-2(P<0.001)、INF-γ(P<0.001)、TGF-β(P<0.001)、IL-6(P<0.001)、IL-7(P<0.001)和CTLA-4(P<0.001)。结论成功构建细胞因子风暴NSG小鼠模型。Objective Establishing CD3 monoclonal antibody to induce humanized cytokine storm mice model.Method The peripheral blood mononuclear cells(PBMCs)of healthy adults were extracted,and NSG mice aged 10-12 weeks after intravenous injection were randomly divided into 2 groups:(1)PBMCs group(2)OKT3(CD3 monoclonal antibody,anti-CD3 mAb,0.1 mg/mL)+PBMCs.The experimental group was injected with CD3 antibody 0.5 mg/kg 6 days later,and serum TGF-β,CTLA-4,IL-10,IL-2,INF-γ,IL-7,and IL-6 levels were detected by ELISA 6 hours later.Result Compared with the control group,the use of OKT3 can effectively induce the cytokine storm IL-2(P<0.001),INF-γ(P<0.001),TGF-β(P<0.001),IL-6(P<0.001),IL-7(P<0.001)and CTLA-4(P<0.001).Conclusion The cytokine storm NSG mouse model constructed this time is conducive to the further study of the disease.

关 键 词：细胞因子风暴 INF-Γ CTLA-4 IL-7 

分 类 号：Q95-3[生物学—动物学]