3种小鼠疼痛模型的对比研究  

Comparative Study of Three Mouse Pain Models

作　　者：郭秋平 陈迪新 黄暨生 贾丹 倪庆纯 GUO Qiuping;CHEN Dixin;HUANG Jisheng;JIA Dan;NI Qingchun(Guangzhou General Pharmaceutical Research Institute Co.,Ltd,Guangzhou 510240,China)

机构地区：[1]广州医药研究总院有限公司,广州510240

出　　处：《实验动物科学》2023年第2期42-48,共7页Laboratory Animal Science

摘　　要：目的对3种小鼠疼痛动物模型进行研究、对比与分析。方法对骨科药物研究中常用小鼠疼痛模型(冰醋酸致小鼠扭体模型、甲醛所致小鼠疼痛模型以及酸引起的慢性疼痛模型)的成模效果和药物镇痛有效性的评价能力进行了对比分析,探讨不同小鼠疼痛模型的疼痛状态模拟能力以及药效评价能力,为镇痛药物研发与评价过程中的模型筛选提供参考。结果在醋酸疼痛模型中,小鼠出现了明显的疼痛反应,其能显著评价神经妥乐平临床剂量10倍稀释剂量下的镇痛作用;甲醛疼痛模型小鼠能明显表现Ⅰ相疼痛反应和Ⅱ相疼痛反应,该模型同样能显著评价神经妥乐平临床剂量10倍稀释剂量下的镇痛作用;小鼠慢性酸疼痛模型表现出更强的镇痛效果评价能力,其能显著评价神经妥乐平临床剂量60倍稀释剂量下的镇痛作用。结论通过对比研究3种不同的疼痛机制模型的疼痛表现和镇痛药效评价能力,小鼠醋酸疼痛模型和甲醛疼痛模型在镇痛能力评价上未表现出明显差别,两者的选用可主要依据需评价药物的镇痛机制进行判断;小鼠慢性疼痛模型对镇痛药物的反应更为敏感,可在镇痛药物剂量摸索研究中优先考虑使用。Objective Research,comparison,and analysis of three mouse pain animal models.Method we established and compared three different analgesic mouse models(acetic acid induced writhing model in mice,formaldehyde induced pain model in mice,and acid induced chronic pain model)to analyze sensitivity,pathological characteristics and drug efficacy evaluating ability.Result In the acetic acid pain model,mice showed significant pain responses,which could significantly evaluate the analgesic effect of neurotropin at 10 times dilution of clinical dose;formaldehyde pain model mice can significantly exhibit both phase I and phase II pain responses,and this model can also significantly evaluate the analgesic effect of neurotropin at 10 times dilution of clinical dose;the mouse model of chronic acid pain exhibits stronger ability to evaluate the analgesic effect,which can significantly evaluate the analgesic effect of neurotropin at 60 times dilution of clinical dose.Conclusion By comparing the pain performance and analgesic efficacy evaluation ability of three different pain mechanism models,the acetic acid pain model and formaldehyde pain model in mice did not show significant differences in analgesic ability evaluation.The selection of the two models can mainly be based on the analgesic mechanism of the drug;the chronic pain model is more sensitive to the response of analgesic drugs and can be prioritized for use in the exploration of analgesic drug dosage.

关 键 词：疼痛模型 对比分析 药物研究 小鼠模型 

分 类 号：Q95-3[生物学—动物学]