2020-2021年中国部分活禽市场中classⅠ类新城疫病毒的分子特征遗传演化和耐热性分析  

Molecular characteristics,genetic evolution and thermostability of class I Newcastle disease virus isolated from partial live bird markets in China from 2020 to 2021

作　　者：张春玮 孙军峰[1] 张智颖 陈林娜 刘添仪 韩宗玺[1] 刘胜旺[1] ZHANG Chun-wei;SUN Jun-feng;ZHANG Zhi-ying;CHEN Lin-na;LIU Tian-yi;HAN Zong-xi;LIU Sheng-wang(Avian Respiratory Infectious Disease Innovation Team,State Key Laboratory of Veterinary Biotechnology/Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150069,China)

机构地区：[1]中国农业科学院,哈尔滨兽医研究所,兽医生物技术国家重点实验室,禽呼吸道传染病创新团队,黑龙江哈尔滨150069

出　　处：《中国兽医科学》2023年第4期491-498,共8页Chinese Veterinary Science

基　　金：国家重点研发计划项目(2021YFD1801105-3);国家蛋鸡产业技术体系岗位科学家项目(CARS-40-K18);国家自然科学基金项目(31872503);黑龙江省自然科学基金团队项目(TD2021C001)。

摘　　要：对2020—2021年期间分离自10个省(自治区)活禽市场不同宿主与部分环境中的新城疫病毒(NDV)进行鉴定,获得了31株classⅠ类NDV。F基因分子特征分析显示,classⅠ类NDV F基因中起始密码子存在突变,导致产生不同长度的编码序列。氨基酸序列分析结果显示,大部分毒株的F蛋白具有典型的弱毒株裂解位点基序,但个别毒株的裂解位点存在特征性的突变。F蛋白中融合肽、N-连接的糖基化位点、中和表位以及七肽重复区域在这31个毒株之间高度保守,而信号肽区域中存在众多的氨基酸替换。HN蛋白中仅N-连接的糖基化位点在31个毒株之间保守,中和表位和七肽重复区域均存在氨基酸替换。此外,首次发现了HN编码区长度为1728 nt的突变毒株。遗传进化分析显示,与其他国家和地区的毒株不同,我国的classⅠ类NDV形成了独特的进化分支,即基因亚型1.1.2。耐热性测定结果显示,classⅠ类NDV分离株表现出不同程度的耐热特性。本研究结果为我国classⅠ类NDV的遗传变异和生物学特性研究奠定了基础。Thirty-one strains of classⅠNewcastle disease virus(NDV)were identified from different hosts and environmental samples from live poultry markets in 10 provinces(autonomous region)between 2020 and 2021.Molecular characteristics analysis of F gene showed that mutations were present at the start codon of F gene of these class I NDVs,resulting in different length of coding sequence.The amino acid sequence analysis demonstrated that the F protein of most strains demonstrated typical cleavage site motifs of avirulent virus,while several strains had characteristic mutations.The fusion peptide,N-linked glycosylation site,neutralizing epitope and heptad repeat regions in F protein were highly conserved among the 31 strains,while there were multiple amino acid substitutions in the signal peptide region.For HN protein,only N-linked glycosylation sites were conserved among the 31 strains,while amino acid substitutions were found in neutralizing epitopes and heptad repeat regions.Besides,a mutant strain with a length of 1728 nt in the HN coding region was identified for the first time.Genetic evolution analysis showed that classⅠNDVs in China formed a unique evolutionary branch in the phylogenetic tree,named genotype 1.1.2,which was distinct from class I NDVs from other countries and regions.The results of thermostability indicated that class I NDV isolates showed different degrees resistance to heat treatment.The results of this study provide insights for the study of genetic variation and biological characteristics of class I NDV in China.

关 键 词：新城疫病毒 分子特征 遗传进化 热稳定性 

分 类 号：S852.659.5[农业科学—基础兽医学]