时间分辨荧光免疫分析的研究进展  

Progress in time-resolved fluorescence immunoassay

作　　者：赵天睿 雷镒妃 许灏钧 张峻豪 匡磊 严蕊 郭爱珍[1,2] 胡长敏[1] ZHAO Tian-rui;LEI Yi-fei;XU Hao-jun;ZHANG Jun-hao;KUANG Lei;YAN Rui;GUO Ai-zhen;HU Chang-min(College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China;State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学动物医学院,湖北武汉430070 [2]华中农业大学农业微生物学国家重点实验室,湖北武汉430070

出　　处：《中国兽医科学》2023年第4期520-525,共6页Chinese Veterinary Science

基　　金：国家重点研发计划项目(2022YFD1800701,2016YFD0500906);湖北省重点研发计划项目(2020BBA055);中央高校基本科研业务费专项(2662020DKPY014);现代农业(肉牛/牦牛)产业技术体系项目(CARS-37)。

摘　　要：时间分辨荧光免疫分析(TRFIA)是一种利用稀土元素荧光特性为基础的检测方法。稀土元素的荧光寿命长且不易被其他荧光影响,使TRFIA具有灵敏度高、特异性强、稳定性好的特点,并在诊断检测领域广泛应用。阐述了TRFIA的原理,并围绕其材料、技术以及与其他检测方法结合应用等方面探讨了TRFIA的最新进展,同时介绍了TRFIA在疾病诊断、食品检测及环境监测的应用,并对其发展前景进行了展望。Time-resolved fluorescence immunoassay(TRFIA)is a detection method based on the fluorescence characteristics of rare earth element.Because rare earth element has a long fluorescence lifetime and is not easily affected by other interfering fluorescence,and TRIFIA has the characteristics of high sensitivity,high specificity and good stability.At present,TRFIA is widely used in the field of diagnosis and detection.This study expounded the principle of TRFIA,and discussed the development of TRFIA in recent years which contained its materials,technology and application in combination with other detection methods.Meanwhile,this study also elaborated the application of TRFIA in disease diagnosis,food detection and environmental monitoring,and prospected to its development.

关 键 词：时间分辨荧光免疫分析 诊断方法 原理 免疫学诊断 

分 类 号：S852.4[农业科学—基础兽医学]