茯苓多糖参与调节BCoV诱导的宿主细胞IFN-β信号通路细胞因子的表达  

Poria cocos polysaccharide involved in regulating the expression of cytokines in host cell β-interferon signaling pathway induced by bovine coronavirus

作　　者：贺泂杰[1] 崔东安[1] 唐豪[2] 王玲[1] 杨峰[1] 王胜义[1] HE Jiong-jie;CUI Dong-an;TANG Hao;WANG Lin;YANG Feng;WANG Sheng-yi(Lanzhou Institute of Husbandry and Pharmaceutical Science,Chinese Academy of Agricultural Sciences,Lanzhou 730050,China;Gansu Provincial Center for Animal Disease Prevention and Control,Lanzhou 730013,China)

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,甘肃兰州730050 [2]甘肃省动物疫病预防控制中心,甘肃兰州730013

出　　处：《中国兽医科学》2023年第4期526-531,共6页Chinese Veterinary Science

基　　金：甘肃省科技重点研发项目(21YF5NA143)。

摘　　要：以牛肾细胞(MDBK)细胞为研究对象,MDBK细胞接种牛冠状病毒(BCoV),应用Real-time PCR检测茯苓多糖对BcoV N基因表达水平及BcoV诱导的MDBK细胞中RLR干扰素信号通路上相关细胞因子表达水平的影响。结果,茯苓多糖可抑制BCoV N基因的表达;茯苓多糖可促进RLR通路细胞因子RIG-I、MDA5、MAVS、TBK1、IRF3及IFN-β的表达,并缓解BCoV对宿主细胞RIG-I、MDA5、MAVS、TBK1、IRF3及IFN-β细胞因子的前期抑制。结果表明,茯苓多糖可调节RLR通路细胞因子表达水平,抑制BCoV病毒的复制。这一研究结果为茯苓多糖治疗BCoV引起的犊牛腹泻提供了理论依据。To study the inhibitory effect of Poria cocos polysaccharide on bovine coronavirus(BCoV),MDBK cells were inoculated with BCoV,the real-time PCR was used to detect the expression level of Poria cocos polysaccharide on BcoV N gene and to analyze the expression level of related cytokines in RLR interferon signaling pathway in BCoV-induced MDBK cells.In result,Poria cocos polysaccharide could inhibit the expression of BCoV N gene.Poria cocos polysaccharide can promote the expression of RIG-I,MDA5,MAVS,TBK1,IRF3 and IFN-βin RLR pathway and alleviate the prophase inhibition of RIG-I,MDA5,MAVS,TBK1,IRF3 and IFN-βin host cells by BCoV.In conclusion,Poria cocos polysaccharide can inhibit the replication of BCoV virus by regulating the expression level of cytokines in RLR pathway,which provide a theoretical basis for the treatment of BCoV-induced calf diarrhea with Poria cocos polysaccharide.

关 键 词：茯苓多糖 犊牛腹泻 牛冠状病毒 细胞因子 BCoV N基因 

分 类 号：S853.74[农业科学—临床兽医学]