益气固表丸含药血清对炎症肺上皮细胞TGF-β_(2)-Smad信号通路的影响  

Effects of Serum Containing Yiqi Gubiao Pills on TGF-β_(2)-Smad Signaling Pathway in Inflammatory Lung Epithelial Cells

作　　者：陶思冥 罗建江[1] 李争[1] 荆晶[1] 孟驰 张艳丽[1] 李风森[1] TAO Siming;LUO Jianjiang;LI Zheng;JING Jing;MENG Chi;ZHANG Yanli;LI Fengsen(Department of Respiratory and Critical Care Medicine,The Fourth Affiliated Clinical Hospital of Xinjiang Medical University,Urumqi 830011,China;Midong Traditional Chinese Medicine Hospital of Urumqi,Urumqi 830011,China)

机构地区：[1]新疆医科大学第四附属临床医院呼吸与危重症医学科,乌鲁木齐830011 [2]新疆乌鲁木齐市米东区中医医院,乌鲁木齐830011

出　　处：《世界中医药》2023年第9期1240-1245,共6页World Chinese Medicine

基　　金：国家自然科学基金项目(81660740);新疆维吾尔自治区自然科学基金青年项目(2019D01C177)。

摘　　要：目的:通过采用益气固表含药血清对慢性阻塞性肺疾病(COPD)炎症细胞模型进行干预,以转化生长因子-β_(2)(TGF-β_(2))-Sma和Mad相关(Smad)信号通路及相关炎症介质为切入点,揭示益气固表丸调节体内炎症水平的可能作用机制。方法:以卷烟烟雾提取物(CSE)和脂多糖(LPS)为刺激因子,以肺上皮细胞A549为载体,建立COPD炎症细胞模型。用益气固表丸对大鼠进行灌胃,2次/d,间隔12 h,给药7 d后采血,制备含药血清。用益气固表丸高、中、低剂量含药血清进行培养干预,运用实时荧光定量PCR法(RT-qPCR)和酶联免疫吸附试验(ELISA)、免疫荧光单标等检测干预前后CD4、CD8、白细胞介素-6(IL-6)、IL-17、肿瘤坏死因子-α(TNF-α)等炎症介质和TGF-β_(2)-Smad信号通路重要分子变化。结果:各给药组CD4显著高于对照组,差异有统计学意义(P<0.05);各给药组CD8、IL-6、IL-17、TNF-α、TGF-β_(2)、Smad2、Smad3表达水平低于对照组,差异有统计学意义(P<0.05)。结论:益气固表丸含药血清对炎症肺上皮细胞的抑制作用可能与调控TGF-β_(2)-Smad信号通路有关。Objective:To reveal the underlying mechanism of Yiqi Gubiao Pills(YQGB)in regulating inflammation in vivo by using YQGB-medicated serum to intervene in the inflammatory cell model of chronic obstructive pulmonary disease(COPD)with the TGF-β_(2)-Smad signaling pathway and related inflammatory factors as the entry point.Methods:The inflammatory cell model of COPD was established with cigarette smoke extract(CSE)and lipopolysaccharide(LPS)as stimulants and lung epithelial A549 cells as the carrier.Rats received YQGB by gavage,twice a day at an interval of 12 h.After 7 days of feeding,blood was collected to prepare drug-containing serum.High-dose,medium-dose,and low-dose YQGB-medicated serum was used for culture intervention.RT-qPCR,ELISA,and immunofluorescence were used to detect the changes of inflammatory cytokines(such as CD4,CD8,IL-6,IL-17,and TNF-α)and important molecules in TGF-β_(2)-Smad signal transduction pathway before and after intervention.Results:CD4 in the groups with drug intervention was significantly higher than that in the control group(P<0.05).The expression levels of CD8,IL-6,IL-17,TNF-α,TGF-β_(2),Smad2,and Smad3 in the groups with drug intervention were lower than those in the control group(P<0.05).Conclusion:The anti-inflammatory effect of YQGB-medicated serum on inflammatory lung epithelial cells may be related to the regulation of the TGF-β_(2)-Smad signaling pathway.

关 键 词：慢性阻塞性肺疾病 益气固表丸 TGF-β_(2)-Smad信号通路 炎症介质 含药血清 

分 类 号：R271.4[医药卫生—中医妇科学]