大豆异黄酮对大鼠脑缺血再灌注损伤的保护作用  

Protective effect of soy isoflavones on rats after cerebral ischemia-reperfusion injury

作　　者：徐建国 谷荣辉 李晒 杜宏洋 李丽[3] 王博文 秦志文 陶泉坊 赵士弟[4] XU Jianguo;GU Ronghui;LI Shai;DU Hongyang;LI Li;WANG Bowen;QIN Zhiwen;TAO Quanfang;ZHAO Shidi(College of Clinical Medicine,Bengbu Medical College,Anhui,Bengbu 233030,China;College of Image,Bengbu Medical College,Anhui,Bengbu 233030,China;Key Laboratory of Basic and Clinical Cardiovascular Diseases,Bengbu Medical College,Anhui,Bengbu 233030,China;Teaching and Research Office of Pathophysiology,Bengbu Medical College,Anhui,Bengbu 233030,China)

机构地区：[1]蚌埠医学院临床医学院,安徽蚌埠233030 [2]蚌埠医学院影像学院,安徽蚌埠233030 [3]蚌埠医学院心脑血管基础与临床重点实验室,安徽蚌埠233030 [4]蚌埠医学院病理生理学教研室,安徽蚌埠233030

出　　处：《中国医药科学》2023年第9期30-33,50,共5页China Medicine And Pharmacy

基　　金：国家级大学生创新创业训练计划立项项目(202110367041);安徽省省级质量工程项目(S202110367120);安徽高校自然科学研究项目(KJ2021ZD0091)。

摘　　要：目的探究大豆异黄酮(SI)对大鼠脑缺血再灌注损伤(CIRI)后的保护作用及机制。方法30只生理状况相同的雄性SD大鼠按照随机数表法分为假手术组(Sham组)、模型组(CIRI组)、大豆异黄酮组(SI组),每组各10只组。除假手术组外其他组采用大鼠大脑中动脉栓塞法(MCAO)建立大鼠脑CIRI模型,SI组造模前进行SI灌胃(120 mg/kg,1次/d,连续21 d)。再灌注24 h后进行神经学功能评分、2,3,5-氯化三苯基四氮唑(TTC)染色、HE染色、微板法检测钙离子(Ca^(2+))浓度,检测超氧化物歧化酶(SOD)活力和丙二醛(MDA)水平。结果造模第二天,CIRI组和Sham组相比大鼠神经功能学评分、脑梗死率、脑组织Ca^(2+)浓度和MDA均有明显升高,SOD活力降低,差异有统计学意义(P<0.05)。SI组与CIRI组相比大鼠神经功能学评分、大鼠脑梗死率、Ca^(2+)浓度、MDA均有明显降低,SOD活力升高,差异有统计学意义(P<0.05)。HE结果显示,Sham组未见脑组织缺血改变,大鼠脑神经细胞排列正常,形态完整,核仁和胞质结构清晰。CIRI组细胞间隙增大组织疏松,排列紊乱,细胞核发生明显固缩,深染。SI组同CIRI组相比,细胞核发生固缩的细胞明显减少,细胞间隙减小。结论SI可能通过抑制氧化应激损伤和降低Ca^(2+)超载对大鼠脑CIRI起保护作用。Objective To investigate the protective effect and mechanism of soy isoflavones(SI)on rats after cerebral ischemia-reperfusion injury(CIRI).Methods A total of 30 male SD rats with the same physiological condition were divided into a Sham-operation group(Sham group,n=10),a model group(CIRI group,n=10),and a SI group(n=10)according to the random number table method.The rat cerebral CIRI model was established by the middle cerebral artery occlusion(MCAO)method in all groups except the Sham-operation group,and the SI group underwent SI gavage(120 mg/kg once daily for 21 d)before modeling.Neurological function rating,and detection of calcium ion(Ca^(2+))concentration,superoxide dismutase(SOD)activity and malondialdehyde(MDA)level by 2,3,5-triphenyltetrazolium chloride(TTC)staining,hematoxylin-eosin(HE)staining and microplate method were performed after 24 h of reperfusion.Results On the second day of modeling,the neurological function score,cerebral infarction rate,Ca^(2+)concentration and MDA in brain tissue of rats were significantly higher and SOD activity was lower in the CIRI group than in the Sham group,with significant differences(P<0.05).The neurological function score,cerebral infarction rate,Ca^(2+)concentration and MDA in brain tissue of rats were significantly lower and SOD activity was higher in the SI group than in the CIRI group,with significant differences(P<0.05).HE staining results showed no ischemic changes in brain tissue,and normally arranged and morphologically intact rat brain neuronal cells with clear nucleoli and cytoplasmic structures in the Sham group.However,HE staining results showed loosely organized and disorderly arranged cells with enlarged cell gaps and significantly pyknosed and deeply stained nuclei in the CIRI group.Besides,compared with the CIRI group,significantly fewer cells with pyknosed nuclei,and reduced cell gaps were very commonly seen in the SI group.Conclusion SI may protect against cerebral CIRI injury in rats by inhibiting oxidative stress injury and reducing Ca^(2+)ove

关 键 词：缺血再灌注损伤 钙超载 大豆异黄酮 氧化应激 神经保护 

分 类 号：R285.5[医药卫生—中药学]