白花蛇舌草化学成分及其抗结肠癌活性初探  

Preliminary Study on the Chemical Constituents of Hedyotis Diffusa and Their Anti-Colon Carcinoma Activity

作　　者：张其爱 杨佳彬[2] 林冠丽 李志健 曾彩芳[2] ZHANG Qiai;YANG Jiabin;LIN Guanli;LI Zhijian;ZENG Caifang(Guangzhou Medical University,Guangzhou,Guangdong,China 511436;The Second Affiliated Hospital of Guangzhou Medical University,Guangzhou,Guangdong,China 511447)

机构地区：[1]广州医科大学,广东广州511436 [2]广州医科大学附属第二医院,广东广州511447

出　　处：《中国药业》2023年第10期46-49,共4页China Pharmaceuticals

基　　金：广州医科大学大学生实验室开放项目[0506309]。

摘　　要：目的分析中药白花蛇舌草40%乙酸乙酯部位的化学成分,并初步探讨其抗结肠癌活性。方法采用中压制备色谱、制备型高效液相色谱对白花蛇舌草40%乙酸乙酯部位进行分离纯化;根据理化性质及波谱数据鉴定化合物结构。采用MTT法检测化合物对结肠癌细胞HT-29和SW480的生长抑制作用。结果共分离得到9个化合物,分别鉴定为齐墩果酸(1)、白桦脂酸(2)、熊果酸(3)、山柰酚(4)、槲皮素(5)、大黄素(6)、大黄酸(7)、芦荟大黄素(8)、大黄酚(9),其中化合物1,2(半数抑制浓度≥100.4,26.5μmol/L)对HT-29和SW480细胞的生长抑制作用较强。结论化合物2,6,7,8为首次从该植物中分离得到,化合物1,2为白花蛇舌草的抗结肠癌活性成分。Objective To analyze the chemical constituents of the 40%ethyl acetate fraction of Hedyotis diffusa,and to study preliminarily their anti-colon carcinoma activity.Methods The medium-pressure liquid chromatography and preparative high-performance liquid chromatography were used to isolate and extract the 40%ethyl acetate fraction of Hedyotis diffusa.The structures of the compounds were dentified based on their physicochemical properties and spectral data.The MTT method was used to detect the inhibitory effect of compounds on the growth of colon carcinoma HT-29 cells and SW480 cells.Results Nine compounds were isolated and identified,including oleanolic acid(1),betulinic acid(2),ursolic acid(3),kaempferol(4),quercetin(5),emodin(6),rhein(7),aloe-emodin(8)and chrysophanol(9).Among them,compounds 1 and 2(half-inhibitory concentration not lower than were 100.4μmol/L and 26.5μmol/L respectively)had a strong inhibitory effect on the growth of HT-29 cells and SW480 cells.Conclusion Compounds 2,6,7 and 8 are isolated from this plant for the first time,and compounds 1 and 2 are the anti-colon carcinoma active constituents of Hedyotis diffusa.

关 键 词：白花蛇舌草 化合物 分离 鉴定 白桦脂酸 齐墩果酸 抗结肠癌活性 

分 类 号：R932[医药卫生—生药学] R284[医药卫生—药学] R285