高效液相色谱法测定复方黄芪健脾口服液中黄芪甲苷含量  

Content Determination of AstragalosideⅣin Compound Huangqi Jianpi Oral Liquid by HPLC

作　　者：王铮 吕益涛 蒋倩倩 滕泽学 强君丽 WANG Zheng;LYU Yitao;JIANG Qianqian;TENG Zexue;QIANG Junli(Baiyin Municipal Center for Drug Inspection and Testing,Baiyin,Gansu,China 730900)

机构地区：[1]甘肃省白银市药品检验检测中心,甘肃白银730900

出　　处：《中国药业》2023年第10期68-71,共4页China Pharmaceuticals

基　　金：甘肃省药品科研项目[2020GSMPA023]。

摘　　要：目的建立测定复方黄芪健脾口服液中黄芪甲苷含量的高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法。方法色谱柱为Agilent 5 HC-C18(2)柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(34∶66,V/V),流速为1.0 mL/min,柱温为40℃,进样量为20μL,蒸发光散射器漂移管温度为40℃,压缩空气压力为350 kPa。结果黄芪甲苷进样量在0.97~9.70μg范围内与峰面积线性关系良好(r=0.9996,n=5);精密度、稳定性、重复性试验结果的RSD均小于2.0%,平均加样回收率为99.78%,RSD为0.98%(n=9)。结论该方法操作简便,结果准确、可靠,重复性好,可用于复方黄芪健脾口服液中黄芪甲苷的含量测定。Objective To establish a high-performance liquid chromatography-evaporative light scattering detector(HPLC-ELSD)method for the content determination of astragalosideⅣin Compound Huangqi Jianpi Oral Liquid.Methods The chromatographic column was Agilent 5 HC-C18(2)column(250 mm×4.6 mm,5μm),the mobile phase was acetonitrile-water(34∶66,V/V),the flow rate was 1.0 mL/min,the column temperature was 40℃,and the injection volume was 20μL.ELSD was adopted,the drift tube temperature was 40℃,and the compressed air pressure was 350 kPa.Results The linear range of astragalosideⅣwas 0.97-9.70μg(r=0.9996,n=5).The RSDs of the precision,stability and repeatability tests were all lower than 2.0%.The average recovery of astragalosideⅣwas 99.78%with an RSD of 0.98%(n=9).Conclusion The method is simple,accurate,reliable and repeatable,which can be used for the content determination of astragalosideⅣin Compound Huangqi Jianpi Oral Liquid.

关 键 词：复方黄芪健脾口服液 高效液相色谱法 蒸发光散射检测器 黄芪甲苷 含量测定 

分 类 号：R917[医药卫生—药物分析学] R927[医药卫生—药学]