体外光化学疗法对大鼠肾移植抗排斥反应的研究  

Research on the antirejection effect of extracorporeal photochemotherapy in rat kidney transplantation

作　　者：周结学[1] 刘东[1] 蒙善东[1] 申升[1] 朱春丽[1] 王晓[1] 庄锦炀 马娟[1] ZHOU Jiexue;LIU Dong;MENG Shandong;SHEN Sheng;ZHU Chunli;WANG Xiao;ZHUANG Jinyang;MA Juan(Department of Organ Transplantation,Guangdong Second Provincial General Hospital,Guangzhou 510317,China)

机构地区：[1]广东省第二人民医院器官移植科,广州510317

出　　处：《实用医学杂志》2023年第8期944-947,共4页The Journal of Practical Medicine

基　　金：广州市科技厅研究基金项目(编号:201804010421)。

摘　　要：目的 探讨输注体外光化学疗法处理的单核细胞对肾移植大鼠排斥反应影响。方法 首先手术建立大鼠肾移植模型,分离受体大鼠单个核细胞,制备8-甲氧基补骨脂素联合紫外线光照的大鼠单核细胞(ECP),根据静脉输注成分不同,将实验分为4组(每组12只),组1接受ECP处理,组2接受传统抗排斥药物,组3接受安慰剂处理,组4非移植组,接受ECP组和安慰剂组受体分别于手术前7 d、手术当天和术后7 d按组别从尾静脉注入ECP细胞(ECP组)或磷酸盐缓冲液PBS(安慰剂组);接受免疫抑制大鼠术后第1天起,皮下注射环孢素A 5 mg/kg连续10 d。组4未接受任何处理。观察受体大鼠的淋巴细胞数、细胞因子变化以及肾功能、移植大鼠肾病理情况。结果 ECP组和免疫抑制剂组大鼠IL-2、INF-γ有不同程度减少、IL-10水平升高,其CD4+、CD8+均有不同程度下降,血肌酐较安慰剂组低,排斥反应程度较安慰剂轻,与安慰剂组相比差异有统计学意义。结论 ECP可调节细胞因子和T细胞,减轻肾移植大鼠的排斥反应。Objective To explore the effect of monocytes treated by extracorporeal photochemotherapy on rejection in rat kidney transplantation.Methods A model of rat kidney transplantation was surgically established.The mononuclear cells from recipient rats were isolated and treated with 8⁃methoxyporalen plus ultraviolet exposure(ECP).The rats were randomly divided into four groups(12 for each group)according to intravenous infusion of different compositions.Group 1 received ECP,group 2 received traditional anti⁃rejection drugs,group 3 received phoaphate buffer solution(PBS,placebo group),and group 4 received no renal transplantation.Group 2 was injected with cyclosporine A of 5 mg/kg subcutaneously from postoperative day 1 for 10 days.The other three groups were injected with ECP,PBS,or normal saline for 7 presurgical days,on the surgical day,and for 7 postsurgical days through the tail vein,respectively.Changes in lymphocyte count,cytokine,renal function and pathology were observed in transplanted mice.Results Levels of IL⁃2,inf⁃γ,CD4^(+),and CD8^(+)were decreased,while IL⁃10 level increased in groups 1 and 2.The degree of rejection was slighter in groups 1 and 2 than in group 3.There were significant differences among the different groups.Conclusions ECP can regulate cytokines and T cells and reduce rejection of kidney transplantation in rats.

关 键 词：体外光化学疗法 肾移植 大鼠 排斥反应 

分 类 号：R617[医药卫生—外科学]