三黄膏对耐甲氧西林金黄色葡萄球菌大鼠皮肤软组织感染的干预作用  

Intervention of Sanhuang ointment on skin and soft tissue infection in methicillin-resistant Staphylococcus aureus rats

作　　者：潘海邦 付琦[2] 王波 王天明 陈乾 李晓丽 吴国泰[4] 郭天康 PAN Hai-bang;FU Qi;WANG Bo;WANG Tian-ming;CHEN Qian;LI Xiao-li;WU Guo-tai;GUO Tian-kang(School of Integrative Traditional Chinese and Western Medicine,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,Gansu Provicne,China;First School of Clinical Medicine,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,Gansu Provicne,China;School of Nursing,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,Gansu Provicne,China;Gansu Provincial Key Laboratory of Traditional Chinese Medicine Pharmacology and Toxicology,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,Gansu Provicne,China;General Surgery Clinical Medicine Center,Gansu Provincial People’s Hospital,Lanzhou 730000,Gansu Province,China)

机构地区：[1]甘肃中医药大学中西医结合学院,甘肃兰州730000 [2]甘肃中医药大学第一临床医学院,甘肃兰州730000 [3]甘肃中医药大学护理学院,甘肃兰州730000 [4]甘肃中医药大学甘肃省中药药理与毒理学重点实验室,甘肃兰州730000 [5]甘肃省人民医院普外临床医学中心,甘肃兰州730000

出　　处：《中国临床药理学杂志》2023年第8期1132-1136,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81860850)。

摘　　要：目的探讨三黄膏对耐甲氧西林金黄色葡萄球菌(MRSA)感染大鼠皮肤软组织的干预作用。方法将96只Wistar大鼠随机分为空白组、模型组、对照组和高、中、低剂量实验组,每组16只。除空白组外,其余大鼠行皮下软组织注射MRSA菌悬液造模。造模成功后,高、中、低剂量实验组分别给予三黄膏1.00、0.50和0.25 g外敷于患处,空白组和模型组均涂抹等量凡士林(1.00 g),对照组给予莫匹罗星0.50 g。6组大鼠每12 h换药1次,连续给药7 d。治疗7 d后处死大鼠,计算创面愈合率,用实时荧光定量聚合酶链反应检测感染组织中白细胞介素-1受体相关激酶1(IRAK-1)和IκB激酶β(IKKβ)mRNA的表达水平,用酶联免疫吸附法检测白细胞介素-6(IL-6)和IL-8的含量。结果治疗7 d后,中、高剂量实验组和对照组、模型组的创面愈合率分别为(88.63±4.54)%、(93.98±3.45)%、(83.02±5.87)%和(60.30±2.30)%,对照组和中、高剂量实验组的创面愈合率与模型组比较,差异均有统计学意义(P<0.05,P<0.01)。高剂量实验组、对照组、模型组和空白组的IRAK-1 mRNA分别为1.14±0.04、1.18±0.05、1.42±0.05和1.01±0.05,IKKβmRNA分别为1.28±0.04、1.30±0.05、1.62±0.04和1.00±0.04,血清IL-6含量分别为(17.83±0.26)、(17.48±0.14)、(19.32±0.42)和(14.51±0.24)pg·mL^(-1),血清IL-8含量分别为(29.04±0.94)、(29.12±0.90)、(33.13±1.01)和(26.37±1.02)pg·mL^(-1)。高剂量实验组和对照组的上述指标与模型组比较,差异均有统计学意义(P<0.05,P<0.01)。结论三黄膏可能通过下调IRAK-1和IKKβ表达,抑制下游炎性分子IL-6和IL-8释放,进而发挥抗炎作用,促进感染创面愈合。Objective To investigate the effect of Sanhuang ointment on skin and soft tissue of rats infected with methicillin-resistant Staphylococcus aureus(MRSA).Methods A total of 96 Wistar rats were randomly divided into blank,model,control and experimental-L,-M,-H groups,with 16 rats per group.Except for the blank group,other rats were injected with MRSA bacteria suspension subcutaneously to make model.After success,experimental-L,-M,-H groups were given Sanhuang ointment 1.00,0.50 and 0.25 g to the affected area.Vaseline 1.00 g of the same amount was applied to the blank and model groups,and the control group was given mupirocin 0.50 g.Six groups of rat s c hanged their dressing every 12 hours for 7 consecutive days.After 7 days of treatment,the rats were killed,the wound healing rate was calculated,and the mRNA expression levels of interleukin-1 receptor-associated kinase 1(IRAK-1)andκB kinaseβ(IKKβ)in infected tissues were detected by real-time fluorescence quantitative polymerase chain reaction,the contents of interleukin-6(IL-6)and IL-8 were detected by enzyme-linked immunosorbent assay.Results After 7 days of treatment,the wound healing rates of the wound healing rates of experimental-L,-M,-H groups,control group and model group were(88.63±4.54)%,(93.98±3.45)%,(83.02±5.87)%and(60.30±2.30)%,respectively.The wound healing rates of the control group,experimental-M,-H groups were significantly different from that of model group(P<0.05,P<0.01).The expression levels of IRAK-1 mRNA in the experimental-H,control,model and blank groups were 1.14±0.04,1.18±0.05,1.42±0.05 and 1.01±0.05;the expression levels of IKKβmRNA were 1.28±0.04,1.30±0.05,1.62±0.04 and 1.00±0.04;the serum IL-6 contents were(17.83±0.26),(17.48±0.14),(19.32±0.42)and(14.51±0.24)pg·mL^(-1);and the serum IL-8 contents were(29.04±0.94),(29.12±0.90),(33.13±1.01)and(26.37±1.02)pg·mL^(-1).Comparing with the model group,differences of the above indexes in the experimental-H and control groups were statistically significant(P<0.05,P<0.01

关 键 词：三黄膏 耐甲氧西林金黄色葡萄球菌 皮肤软组织感染 Toll样受体/核因子κB信号通路 白细胞介素-1受体相关激酶1 IκB激酶β 

分 类 号：R28[医药卫生—中药学]