猪圆环病毒体外高效增殖研究进展  

Research advance on efficient replication of porcine circovirus in vitro

作　　者：雷白时[1,2] 薛拥志 李秀丽[1] 包银莉[4] 张武超 赵款 袁万哲 LEI Baishi;XUE Yongzhi;LI Xiuli;BAO Yinli;ZHANG Wuchao;ZHAO Kuan;YUAN Wanzhe(College of Veterinary Medicine,Hebei Agricultural University,Baoding,Hebei 071001,China;Hebei Key Laboratory of Analysis and Control of Zoonotic Pathogenic Microorganism,Baoding,Hebei 071001,China;College of Animal Science and Technology,Hebei North University,Zhangjiakou,Hebei 075131,China;Key Laboratory of Fujian Universities Preventive Veterinary Medicine and Biotechnology,Longyan University,Longyan,Fujian 364012,China)

机构地区：[1]河北农业大学动物医学院,河北保定071001 [2]河北省人畜共患病原微生物分析与防控重点实验室,河北保定071001 [3]河北北方学院动物科技学院,河北张家口075131 [4]龙岩学院预防兽医学与生物技术福建省高等学校重点实验室,福建龙岩364012

出　　处：《中国兽医学报》2023年第4期812-817,共6页Chinese Journal of Veterinary Science

基　　金：中央引导地方科技发展资金资助项目(226Z6602G);河北省省属高等学校基本科研业务费资助项目(KY202017);福建省家畜传染病防治与生物技术重点实验室开放基金资助项目(2021ZDKF03);预防兽医学与生物技术福建省高等学校重点实验室2020年度开放课题资助项目(2020KF03)。

摘　　要：猪圆环病毒相关疾病(porcine circovirus diseases,PCVDs)主要通过疫苗产品进行预防,病毒增殖水平与疫苗研发密切相关,随着新发病原PCV3和PCV4的出现和广泛流行,对于实现此类病毒体外高效增殖,已经成为开展相应病毒病原学、发病机制及其疫苗研究方面的瓶颈。鉴于4种猪圆环病毒(PCV)在基因组结构、复制机制等方面的相似性,现对其中已实现体外培养的PCV2,在促进其病毒增殖方面的方法和研究进展进行总结和概括,以期进一步提升现有PCV2增殖水平,并对实现PCV3或PCV4的体外培养提供助力,从而促进PCV疫苗的研发和相关病原学与发病机制的深入研究。Porcine circovirus diseases(PCVDs)are mainly prevented through vaccine products.The level of virus replication is closely related to vaccine research and development.With the emergence and widespread prevalence of new pathogens PCV3and PCV4,it has become a bottleneck in the research of corresponding virus etiology,pathogenesis and vaccinology to realize the efficient replication of this kind of virus in vitro.In view of the similarities of the four porcine circoviruses in genome structure and replication mechanism,methods and research progress of promoting virus replication of PCV2achieved in vitro culture are summarized in this paper to further improve the existing PCV2replication level,provide assistance for the realization of PCV3or PCV4cultured in vitro,and promote the research and development of porcine circovirus etiology,pathogenesis and vaccine technology.

关 键 词：猪圆环病毒 体外培养 病毒增殖 研究进展 

分 类 号：S852.65[农业科学—基础兽医学]