Mepanipyrim induces visual developmental toxicity and vision-guided behavioral alteration in zebrafish larvae  

作　　者：Chao Shen Yimei Cai Jialing Li Chengyong He Zhenghong Zuo 

机构地区：[1]State Key Laboratory of Cellular Stress Biology,School of Life Sciences,Xiamen University,Xiamen 361005,China [2]State Key Laboratory of Marine Environmental Science,Xiamen University,Xiamen 361005,China

出　　处：《Journal of Environmental Sciences》2023年第2期76-88,共13页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No.42177411);the Natural Science Foundation of Fujian Province of China (No.2018J01067)

摘　　要：Mepanipyrim,an anilinopyrimidine fungicide,has been extensively used to prevent fungal diseases in fruit culture.Currently,research on mepanipyrim-induced toxicity in organisms is still very scarce,especially visual developmental toxicity.Here,zebrafish larvae were employed to investigate mepanipyrim-induced visual developmental toxicity.Intense light andmonochromatic light stimuli-evoked escape experiments were used to investigate vision-guided behaviors.Meanwhile,transcriptomic sequencing and real-time quantitative PCR assays were applied to assess the potential mechanisms of mepanipyrim-induced visual developmental toxicity and vision-guided behavioral alteration.Our results showed that mepanipyrim exposure could induce retinal impairment and vision-guided behavioral alteration in larval zebrafish.In addition,the grk1b gene of the phototransduction signaling pathway was found to be a potential aryl hydrocarbon receptor(AhR)-regulated gene.Mepanipyrim-induced visual developmental toxicity was potentially related to the AhR signaling pathway.Furthermore,mepanipyrim-induced behavioral alteration was guided by the visual function,and the effects of mepanipyrim on long and middle wavelength light-sensitive opsins may be the main cause of vision-guided behavioral alteration.Our results provide insights into understanding the relationship between visual development and vision-guided behaviors induced by mepanipyrim exposure.

关 键 词：Danio rerio mepanipyrim visual developmental toxicity vision-guided behaviors phototransduction signaling pathway grk1b 

分 类 号：X503.225[环境科学与工程—环境工程]