Repeated hydrogen peroxide dosing briefly reduces cyanobacterial blooms and microcystin while increasing fecal bacteria indicators in a eutrophic pond  

作　　者：Mark W.Lusty Christopher J.Gobler 

机构地区：[1]School of Marine and Atmospheric Sciences,Stony Brook University,Southampton,NY 11968,USA

出　　处：《Journal of Environmental Sciences》2023年第2期522-543,共22页环境科学学报（英文版）

基　　金：funded by the New York State Department of Environmental Conservation, the Tamarind Foundation, and NOAA’s MERHAB program, publication #237

摘　　要：This study explored the effects of H_(2)O_(2)on Cyanobacteria and non-target microbes using fluorometry,microscopy,flow cytometry,and high throughput DNA sequencing of the 16S rRNA gene during a series of mesocosm and whole-ecosystem experiments in a eutrophic pond in NY,USA.The addition of H_(2)O_(2)(8 mg/L)significantly reduced Cyanobacteria concentrations during a majority of experiments(66%;6 of 9)and significantly increased eukaryotic green and unicellular brown algae in 78%and 45%of experiments,respectively.While heterotrophic bacteria declined significantly following H_(2)O_(2)addition in all experiments,bacteria indicative of potential fecal contamination(Escherichia coli,Enterococcus,fecal coliform bacteria)consistently and significantly increased in response to H_(2)O_(2),evidencing a form of‘pollution swapping’.H_(2)O_(2)more effectively reduced Cyanobacteria in enclosed mesocosms compared to whole-ecosystem applications.Ten whole-pond H_(2)O_(2)applications over a twoyear period temporarily reduced cyanobacterial levels but never reduced concentrations below bloom thresholds and populations always rebounded in two weeks or less.The bacterial phyla of Cyanobacteria,Actinobacteria,and Planctomycetes were the most negatively impacted by H_(2)O_(2).Microcystis was always reduced by H_(2)O_(2),as was the toxin microcystin,but Microcystis remained dominant even after repeated H_(2)O_(2)treatments.Although H_(2)O_(2)favored the growth of eukaryotic algae over potentially harmful Cyanobacteria,the inability of H_(2)O_(2)to end cyanobacterial blooms in this eutrophic waterbody suggests it is a non-ideal mitigation approach in high biomass ecosystems and should be used judiciously due to potential negative impacts on non-target organisms and promotion of bacteria indicative of fecal contamination.

关 键 词：Indicator bacteria Toxic cyanobacteria MICROCYSTIN MICROCYSTIS Hydrogen peroxide Bacteria ENTEROCOCCUS E.coli Pollution swapping 

分 类 号：V52[航空宇航科学与技术—人机与环境工程]