益气通阳颗粒对免疫性血小板减少症模型小鼠的治疗作用及机制  

Therapeutic effect and mechanism of Yiqi Tongyang Granule in treating immune thrombocytopenia model mice

作　　者：杨秀鹏[1] 许勇钢[1] 麻柔[1] 杜宇 彭勍[1] 王洪志[1] 蔡晓青[1] 魏丢 李芮 王德秀 李柳[1] YANG Xiupeng;XU Yonggang;MA Rou;DU Yu;PENG Qing;WANG Hongzhi;CAI Xiaoqing;WEI Diu;LI Rui;WANG Dexiu;LI Liu(Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China)

机构地区：[1]中国中医科学院西苑医院,北京100091

出　　处：《北京中医药大学学报》2023年第4期502-508,共7页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然基金面上项目(No.81072928);北京市科学技术委员会“十病十药”专项课题项目(No.Z161100001816019)。

摘　　要：目的探讨益气通阳颗粒对免疫性血小板减少症模型小鼠的治疗作用及作用机制。方法以BALB/c小鼠为研究对象,腹腔注射豚鼠抗小鼠血小板血清建立免疫性血小板减少症小鼠模型。造模小鼠随意分为模型组、益气通阳组、金薯叶组、泼尼松组,每组10只;10只未造模小鼠为对照组。各治疗组分别以益气通阳颗粒(17.4 g/kg)、酸酸泼尼松片(9.1 mg/kg)和金薯叶止血合剂(3.9 mL/kg),灌胃11 d。检测各组小鼠的外周血血小板(PLT)、白细胞(WBC)计数和血红蛋白(Hb)含量;通过胸骨骨髓涂片和骨髓病理比较各组的骨髓巨核细胞总数,以及不同类型巨核细胞的比例;检测小鼠脾脏和胸腺的脏器系数,观察脾脏和胸腺的病理改变;流式细胞术检测小鼠淋巴细胞亚群。结果与对照组相比,模型组小鼠PLT计数减少(P<0.05),经益气通阳颗粒、泼尼松和金薯叶止血合剂治疗后PLT计数增加(P<0.05)。与对照组相比,模型组小鼠原始和幼稚巨核细胞数量增加、产板巨核细胞比例减少(P<0.05),经益气通阳颗粒治疗后原始和幼稚巨核细胞比例减少,而产板巨核细胞比例增加(P<0.05)。病理观察发现,模型组脾脏增大,可见较多髓外造血,各治疗组与模型组相比脾脏减小且髓外造血现象减少;经泼尼松治疗的免疫性血小板减少症小鼠胸腺萎缩。流式细胞术检测发现,与对照组相比,模型组小鼠细胞毒性T细胞(Tc)、辅助性T细胞(Th)、调节性T细胞(Treg)细胞百分比降低,经益气通阳颗粒治疗后升高(P<0.05)。结论益气通阳颗粒可通过调控淋巴细胞亚群调节免疫性血小板减少症小鼠的免疫状态,通过影响骨髓产板巨核细胞的数量促进外周血PLT升高,其疗效与泼尼松相近,明显优于金薯叶止血合剂。Objective To explore the therapeutic effect of Yiqi Tongyang Granule in the treatment of immune thrombocytopenia mice and the underlying mechanism.Methods BALB/c mice were treated with anti-mouse platelet serum from guinea pig to establish an immune thrombocytopenia mouse model.Model mice were randomly devided into the model group,the prednisone group,the Yiqi Tongyang Granule group,and the Jinshuye group;10 unmodeled mice as the control group.The mice were treated with Yiqi Tongyang Granule(17.4 g/kg),prednisone(9.1 mg/kg),and Jinshuye Styptic Mixture(3.9 mL/kg)by gavage for 11 days,and the platelets(PLT),white blood cells(WBC),and hemoglobin(Hb)counts in the mice were measured.Bone marrow megakaryocyte counts were compared by bone marrow smears and bone marrow pathology,and the proportions of different types of megakaryocytes in bone marrow were analyzed.The organ coefficients and pathological changes of the spleen and thymus were observed in mice.Lymphocyte subsets were detected using a flow cytometer.Results Compared with the control group,the number of PLT in immune thrombocytopenia mice was reduced(P<0.05),and after treatment with Yiqi Tongyang Granule,prednisone,and Jinshuye Styptic Mixture,the number of PLT was increased(P<0.05).Compared with the control group,the number of bone marrow megakaryocytes was increased and the proportion of PLT-producing megakaryocytes was decreased in immune thrombocytopenia mice(P<0.05).The number of megakaryocytes was decreased after treatment with Yiqi Tongyang Granule(P<0.05),the ratio of primitive and naive megakaryocytes was decreased,and the proportion of PLT-producing megakaryocytes was increased in the immune thrombocytopenia mice(P<0.05).The spleen was observed to be enlarged in the immune thrombocytopenia mice based on the spleen organ coefficient and pathology,and extramedullary hematopoiesis was found to be common in the spleens of immune thrombocytopenia mice.Thymus atrophy was very obvious in immune thrombocytopenia mice treated with prednisone based on the thy

关 键 词：免疫性血小板减少症 益气通阳颗粒 淋巴细胞亚群 小鼠 

分 类 号：R285.5[医药卫生—中药学]