金雀异黄素诱导乳腺癌细胞线粒体损伤和G2/M细胞周期阻滞的作用  

Effects of genistein on mitochondrial damage and G2/M phase arrest in breast cancer cells

作　　者：产柳佳 王文敬 逄雨衡 邹新 易冰 王玉记[4] 赵立春[1] CHAN Liujia;WANG Wenjing;PANG Yuheng;ZOU Xin;YI Bing;WANG Yuji;ZHAO Lichun(Guangxi University of Chinese Medicine,Nanning 530200,China;Beijing Institute of Hepatology,Beijing 100069,China;Harbin Medical University Cancer Hospital,Harbin 150081,China;Capital Medical University,Beijing 100069,China)

机构地区：[1]广西中医药大学,南宁530200 [2]北京市肝病研究所 [3]哈尔滨医科大学附属肿瘤医院 [4]首都医科大学

出　　处：《北京中医药大学学报》2023年第4期520-527,共8页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(No.82072903);广西自然科学基金面上项目(No.2020GXNSFAA238021);广西自然科学基金重点项目(No.2021GXNSFDA075008)。

摘　　要：目的 研究金雀异黄素对乳腺癌细胞株线粒体损伤和G2/M细胞周期阻滞的作用。方法 采用细胞增殖与活性检测-8(CCK-8)法检测金雀异黄素(12.5、25、50、100、150、200μmol/L)作用48 h对MDA-MB-231和MCF-7细胞的增殖影响;碘化丙啶单染法检测金雀异黄素(50、100、150μmol/L,48 h)对MDA-MB-231和MCF-7细胞周期的影响;JC-1、MitoTracker Orange CMTMRos和Hoechst 33342染色法检测金雀异黄素(50、100、150μmol/L,48 h)对MDA-MB-231和MCF-7细胞线粒体功能及细胞凋亡的影响。结果 与对照组相比,金雀异黄素给药组细胞增殖活力下降,不同浓度金雀异黄素对细胞增殖均有抑制作用(P<0.05,P<0.01);金雀异黄素可诱导MDA-MB-231和MCF-7细胞发生凋亡,并将细胞周期阻滞在G2/M期(P<0.05,P<0.01);金雀异黄素可有效降低MDA-MB-231和MCF-7细胞线粒体膜电位,并降低线粒体活性(P<0.05,P<0.01)。结论 金雀异黄素可以抑制MDA-MB-231和MCF-7细胞的增殖,并使其周期阻滞在G2/M期,诱导细胞凋亡,其作用机制可能与诱导线粒体功能损伤有关。Objective We aimed to study the effect of genistein on mitochondrial damage and G2/M cell cycle arrest in breast cancer cell strain.Methods The Cell Counting Kit-8(CCK-8)assay was used to determine the proliferation of MDA-MB-231 and MCF-7 cells after administration of genistein(12.5,25,50,100,150,and 200μmol/L)for 48 h.The effects of genistein(50,100,and 150μmol/L for 48 h)on the cell cycle were detected by PI staining of MDA-MB-231 and MCF-7 cells.JC-1,MitoTracker Orange CMTMRos,and Hoechst 33342 staining assays were used to detect the effects of genistein(50,100,and 150μmol/L for 48 h)on mitochondrial function and apoptosis in MDA-MB-231 and MCF-7 cells.Results Compared with the control group,the cell proliferation activity was decreased in genistein groups,genistein could inhibit the proliferation of cells in each concentration(P<0. 05,P<0. 01). Genistein induced nuclear chromatin agglutination in MDA-MB-231 and MCF-7 cells,which indicated that apoptosis was promoted. Genistein arrested the cell cycle of MDA-MB-231 andMCF-7 cells in the G2/ M phase (P<0. 05, P<0. 01). Genistein decreased the mitochondrial membranepotential and mitochondrial activity in MDA-MB-231 and MCF - 7 cells ( P < 0. 05, P < 0. 01).Conclusion Genistein could inhibit the proliferation of MDA-MB-231 and MCF-7 cells, induce cellcycle arrest in the G2/ M phase, and induce apoptosis. The underlying mechanisms could be related withmitochondrial function modulation.

关 键 词：金雀异黄素 乳腺癌细胞株 线粒体 细胞周期 

分 类 号：R285.5[医药卫生—中药学]