阿维菌素纳米微囊悬浮剂的高效液相色谱分析方法建立与优化  

Establishment and optimization of determination of abamectin nanocapsule suspension by HPLC

作　　者：曹国发 徐源 唐春慧 孙香莹 张振博 张静姝[1,2] 王玉邦 CAO Guo-fa;XU Yuan;TANG Chun-hui;SUN Xiang-ying;ZHANG Zhen-bo;ZHANG Jing-shu;WANG Yu-bang(School of Public Health,Nanjing Medical University,Nanjing 211166,China;Safety Assessment and Research Center for Drug,Pesticide and Veterinary Drug of Jiangsu Province,Nanjing 211166,China)

机构地区：[1]南京医科大学公共卫生学院,南京211166 [2]江苏省医药农药兽药安全性评价与研究中心,南京211166

出　　处：《农药》2023年第5期334-336,共3页Agrochemicals

摘　　要：[目的]利用高效液相色谱(HPLC)建立阿维菌素纳米微囊悬浮剂中有效成分阿维菌素的检测方法,并进行优化。[方法]样品经乙腈超声进行破囊提取,采用HPLC法,以乙腈-0.1%甲酸水溶液(体积比90∶10)为流动相,使用XDB-C18反相色谱柱和二极管阵列检测器(DAD),在245 nm波长下对样品中的阿维菌素进行测定,外标法定量。[结果]方法的线性相关系数为0.9999;变异系数为0.81%;平均回收率为98.44%。[结论]该检测方法快速、简便,准确度和精密度高,可用于阿维菌素纳米微囊悬浮剂的定量分析。[Aims] This study aimed to establish an analytical method for detecting abamectin in nanocapsule suspension by high performance liquid chromatography(HPLC)and optimization of it.[Methods] The samples were extracted with acetonitrile by ultrasound from broken capsules.The abamectin in the samples was determined by HPLC with DAD detector and XDB-C18reversed-phase column at 245 nm while acetonitrile-0.1% formic acid aqueous solution(90:10,by vol)was used as the mobile phase,and quantified by external standard method.[Results] The results showed that the linear correlation coefficient of abamectin was 0.9999 and the coefficient of variation was 0.81%.The average recovery was 98.44%.[Conclusions] This method is rapid,simple,accurate and precise,which could be applied for quantitative analysis of abamectin nanocapsule suspension.

关 键 词：阿维菌素 纳米微囊 高效液相色谱 

分 类 号：TQ450.7[化学工程—农药化工]