微核糖核酸簇miR-290-295促进体细胞重编程  

MicroRNA Cluster 290-295 Enhances Somatic Cell Reprogramming

作　　者：朱思颖 杨洋[2] 李鹏东 薛燕婷 佘芹[2] 齐玲 赵国军 廖宝剑 ZHU Si-ying;YANG Yang;LI Peng-dong;XUE Yan-ting;SHE Qin;QI Ling;ZHAO Guo-jun;LIAO Bao-jian(College of Pharmacy,Dali University,Dali 671000,China;The Sixth Affiliated Hospital of Guangzhou Medical University,Qingyuan 511518,China;Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou 510530,China)

机构地区：[1]大理大学药学院,大理671000 [2]广州医科大学附属第六医院,清远511518 [3]中国科学院广州生物医药与健康研究院,广州510530

出　　处：《中国生物工程杂志》2023年第4期1-9,共9页China Biotechnology

基　　金：广东省自然科学基金(2021A15220179)。

摘　　要：目的:探究干细胞中表达丰度最高的微核糖核酸簇miR-290-295对体细胞重编程的影响。方法:使用逆转录病毒载体将miR-290-295簇在小鼠体细胞中过表达,研究其促进体细胞重编程为诱导性多能干细胞(induced pluripotent stem cells,iPSCs)以及此过程对细胞功能的影响。结果:miR-290-295簇的过表达在三因子(Sox2、Klf4、Oct4)诱导体系中能够显著提高小鼠体细胞重编程的效率;过表达miR-290-295簇能够促进重编程中多能性标记基因的上调与体细胞标记基因的下调,同时也会促进间质-上皮细胞转化(mesenchymal-epithelial transition,MET)标记基因的表达。结论:miR-290-295簇对小鼠体细胞重编程具有促进作用,这有助于深入理解干细胞多能性和重编程的RNA调节机制,为开发新型诱导体系提供了新视角。Objective:To explore the effect of miR-290-295,the most abundantly expressed microRNA cluster in stem cells,as a whole on somatic cell reprogramming.Methods:The miR-290-295 cluster was overexpressed into mouse somatic cells using retroviral vectors to explore whether it promotes the reprogramming of somatic cells into induced pluripotent stem cells(iPSCs)and the effect on cell function through this process.Results:The overexpression of miR-290-295 cluster could significantly improve the efficiency of mouse somatic cell reprogramming in the traditional induction system of three factors(Sox2,Klf4 and Oct4).Overexpression of miR-290-295 cluster could not only promote the up-regulation of pluripotency marker genes and the down-regulation of somatic marker genes during reprogramming,but also the expression of mesenchymal-epithelial transition(MET)marker genes and cell proliferation related genes.Conclusion:miR-290-295 promotes the reprogramming of mouse somatic cells.Our findings are helpful to understand the RNA regulatory mechanism in stem cell pluripotency and reprogramming,and provide a new perspective for the development of new induction systems.

关 键 词：体细胞重编程 诱导性多能干细胞 微核糖核酸 miR-290-295簇 

分 类 号：Q813[生物学—生物工程]