白藜芦醇减轻直肠癌放疗大鼠肠道损伤效果及作用机制研究  

Resveratrol attenuates intestinal injury in rectal cancer radiotherapy rats by regulating PI3K/Akt/mTOR protein pathway by inhibiting ROS and inflammatory response

作　　者：王永霞 樊捷婷 武晓媛 刘戴维 胡嘉芮 何晓华[1] 武雪亮[2] 李占林[1] WANG Yong-xia;FAN Jie-ting;WU Xiao-yuan;LIU Dai-wei;HU Jia-rui;HE Xiao-hua;WU Xie-liang;LI Zhan-lin(Department of Traditional Chinese Medicine,First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei Province 075000,China;General Surgery Department of the First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei Province 075000,China)

机构地区：[1]河北北方学院附属第一医院中医科,河北张家口075000 [2]河北北方学院附属第一医院普通外科,河北张家口075000

出　　处：《解剖学研究》2023年第2期127-132,共6页Anatomy Research

基　　金：河北省卫计委课题(20180834)。

摘　　要：目的探讨白藜芦醇对直肠癌放疗大鼠肠道损伤的影响及其作用机制。方法研究对象选取健康大鼠18只,均建立直肠癌模型。建立后随机均分为对照组及白藜芦醇组,每组9只,对照组给予安慰剂,白藜芦醇组给予20 mg·kg^(-1)·d^(-1)白藜芦醇,两组均给予单剂量5Gy放疗。连续干预3周后比较两组大鼠氧化应激(ROS)标志物、炎性因子介质表达水平;观察两组大鼠肠道病理组织学变化,使用TUNEL染色观察两组肠道绒毛、隐窝细胞凋亡;使用蛋白印迹检测两组大鼠PI3K、AKT、m TOR蛋白表达。结果白藜芦醇组MDA水平显著低于对照组,HSH、CAT水平显著高于对照组,差异有统计学意义(P<0.05);白藜芦醇组TNF-α、NF-κB、IL-1β水平显著低于对照组,差异有统计学意义(P<0.05);白藜芦醇组绒毛、隐窝细胞凋亡率低于对照组,差异有统计学意义(P<0.05);白藜芦醇组PI3K、Akt和mTOR蛋白相对表达量显著低于对照组,差异有统计学意义(P<0.05);病理组织学检测显示,对照组肠黏膜部分绒毛尖端坏死,固有层白细胞炎症细胞浸润,绒毛高度和宽度杂乱,部分绒毛可见缩短、萎缩,增生和多动杯状细胞出现,出现肠道损伤,白藜芦醇组肠道损伤缓解,肠道组织结构改善。结论白藜芦醇通过抑制PI3K/Akt/mTOR通路,在改善放疗诱导的ROS产生和炎症方面具有潜在的治疗价值,可减轻放疗大鼠肠道炎症并减少大鼠肠道绒毛、隐窝细胞凋亡。白藜芦醇可能可作为一种有前景的放疗辅助剂,通过减少放疗后的肠道并发症来提高治疗效果和安全性。Objective To explore the effect of resveratrol on intestinal injury in rats with rectal cancer radiotherapy and its mechanism.Methods All 18 healthy rats were selected to establish rectal cancer model.After establishment,all patients were divided into control group and resveratrol group,with 9 patients in each group.The control group was given placebo,the resveratrol group was given resveratrol 20 mg kg^(-1)·d^(-1),and both groups were given a single dose of 5 Gy radiotherapy.After 3 weeks of continuous intervention,the expression levels of oxidative stress(ROS)markers and inflammatory factor mediators were compared between the two groups.The intestinal histopathological changes of rats in two groups were observed,and the apoptosis of intestinal vllus and crypt cells in two groups was observed by TUNEL staining,The expressions of PI3K,AKT and mTOR were detected by Western blot.Results The MDA level of the resveratrol group was significantly lower than that of the control group,and the HSH and CAT levels were significantly higher than those of the control group,with statistical significance(P<0.05).The levels of TNF-α,NF-kB and IL-1βin the resveratrol group were significantly lower than those in the control group,and the difference was statistically significant(P<0.05).The apoptosis rate of vilus and crypt cells in the resveratrol group was lower than that in the control group,and the diference was statistically significant(P<0.05).The relative protein expressions of PI3K,Akt and mTOR in the resveratrol group were significantly lower than those in the control group with statistical significance(P<0.05).Histopathological examination showed that part of the intestinal mucosa in the control group was necrotic at villus tip,infiltration of leukocyte inflammatory cells in lamina propria,disorganized villi in height and width,shortening and atrophy,hyperplasia and hyperactive goblets appeared in part of the villi,and intestinal damage was observed.In the resveratrol group,intestinal damage was relieved and intestinal

关 键 词：白藜芦醇 直肠癌 放疗 氧化应激 炎症 肠道损伤 

分 类 号：R285.5[医药卫生—中药学]