增液八珍汤对慢传输型便秘大鼠模型结肠肌5-HT、AchE、SP及VIP的影响  

Effects of Zengye Bazhen Decoction on 5-HT,AchE,SP and VIP in Colon in Rat Model of STC

作　　者：陈泽芃 王辰龙 顾庆龙 陈瑞超 CHEN Zepeng;WANG Chenlong;GU Qinglong;CHEN Ruichao(Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China;Xuzhou Medical University,Xuzhou 221004,China;Xuzhou Hospital of Traditional Chinese Medicine,Xuzhou 221000,China)

机构地区：[1]南京中医药大学附属医院,江苏南京210029 [2]徐州医科大学,江苏徐州221004 [3]江苏省徐州市中医院,江苏徐州221000

出　　处：《中医药信息》2023年第5期21-26,共6页Information on Traditional Chinese Medicine

基　　金：徐州市应用基础研究计划项目(KC18042)。

摘　　要：目的:观察增液八珍汤对慢传输型便秘大鼠结肠肌间神经丛中5-HT、AchE、VIP、SP的影响。方法:将72只SPF级大鼠随机分为空白对照组、模型组、麻仁软胶囊组、增液八珍汤低剂量组、增液八珍汤中剂量组和增液八珍汤高剂量组各12只。麻仁软胶囊组给予麻仁软胶囊内容物6.2 mL/(kg·d)灌胃,增液八珍汤低、中、高剂量组分别给予17、34、68 g/(kg·d)增液八珍汤灌胃,共给药14 d,空白对照组和模型组给等量的生理盐水。大鼠结肠组织给予HE染色及免疫组化染色,并对染色组化结果进行半定量积分分析。结果:与模型组比较,增液八珍汤各剂量组及麻仁软胶囊组结肠肌间神经丛神经元阳性表达和5-HT、AchE、VIP、SP定量分析显著增加(P<0.05);与麻仁软胶囊组比较,增液八珍汤各剂量组及麻仁软胶囊组结肠肌间神经丛神经元阳性表达和5-HT、AchE、VIP、SP定量分析显著增加(P<0.05),增液八珍汤中、高剂量组对结肠肌间神经丛5-HT、AchE、VIP、SP神经元增加的量均优于麻仁软胶囊组(P<0.05)。结论:增液八珍汤可通过增加便秘大鼠结肠中5-HT、AchE、VIP、SP表达神经细胞含量,刺激结肠收缩,增加结肠动力,加快结肠传导速度。Objective:To observe the effects of Zengye Bazhen Decoction on serotonin(5-HT),acetylcholinesterase(AchE),vasoactive intestinal peptide(VIP)and substance P(SP)in colonic intermuscular plexus of rats with slow-transit constipation(STC).Methods:72 SPF rats were randomly divided into the blank group,the model group,the Maren Soft Capsule group,the Zengye Bazhen Decoction groups of low-dose(17 g/kg·d),medium-dose(34 g/kg·d)and high-dose(68 g/kg·d),with 12 rats in each group.The Maren Soft Capsule group was treated with Maren Soft Capsule at the dose of 6.2 mL(/kg·d),and the Zengye Bazhen Decoction groups were treated with Zengye Bazhen Decoction at different doses of 17 g(/kg·d),34 g(/kg·d)and 68 g(/kg·d).The blank group and the model group were given the same amount of normal saline.The duration of interventions were 14 days.Rat colon tissues were subjected to HE staining and immunohistochemical staining,and semi-quantitative integral analysis was performed on the staining and histochemical results.Results:Compared with those in the model group,the positive neuron expressions of 5-HT,AchE,VIP and SP in the colonic intermuscular plexus were increased significantly in the Zengye Bazhen Decoction groups and the Maren Soft Capsule group(P<0.05).Compared with those in the Maren Soft Capsule group,the positive neuron expressions of 5-HT,AchE,VIP and SP in the colonic intermuscular plexus were increased significantly in the Zengye Bazhen Decoction groups(P<0.05),and the amount of increased neurons of 5-HT,ACHE,VIP and SP were more significant in the Zengye Bazhen Decoction groups of medium-dose and high-dose than that in the Maren Soft Capsule group(P<0.05).Conclusion:Zengye Bazhen Decoction can stimulate colonic contraction,increase colonic motility and accelerate colonic transmission speed by increasing the neuron expressions of 5-HT,AchE,VIP and SP in the colon of constipated rats.

关 键 词：增液八珍汤 慢传输型便秘 5-HT ACHE SP VIP 结肠肌间神经丛 

分 类 号：R285.5[医药卫生—中药学]